| Alternative to fluorescence assays to monitor fusion between Acholeplasma laidlawii cells and liposomes. | |
| | |
MedLine Citation:
|
PMID: 12460438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIMS: To develop a new technique as an alternative to the fluorescence assays and electron microscopy for the purpose of monitoring the cell-liposome fusion. METHODS AND RESULTS: Acholeplasma laidlawii whole cells did not oxidize Glucose-6-phosphate (G6P) or Fructose-1,6 diphosphate (F1,6DP) as free (unentrapped) substrates, at concentrations 47 and >270 mM, respectively. Lysed A. laidlawii cells oxidized G6P and F1,6DP at lower concentration of 0.8 and 15 mM, respectively. When these substrates were entrapped inside liposomes, at a final concentration of 1.5 mM, and interacted with A. laidlawii whole cells, in an oxygen electrode chamber, an increase in oxygen uptake was evident. This interaction does not have any effect on cell viability. SIGNIFICANCE AND IMPACT OF THE STUDY: The experimental system described here is advantageous over classical fluorescence assays in determining the fate of liposome-entrapped material and raises the possibility of studying the kinetics of metabolic substrates, which are normally excluded from the cell by the cell membrane. |
| | |
Authors:
|
K K Abu-Amero; M A Halablab; R J Miles |
Related Documents
:
|
2834888 - Cellular spin resonance of yeast in a frequency range up to 140 mhz. 186868 - Vertebrate central nervous system: same neurons mediate both electrical and chemical in... 9498588 - Gradient, high-resolution, magic angle spinning 1h nuclear magnetic resonance spectrosc... 17828458 - Electrophoresis-assisted single-cell electroporation for efficient intracellular delivery. 11240668 - Regulation of cell growth and hpv genes by exogenous estrogen in cervical cancer cells. 605388 - Cybrid formation in mouse l cells: the influence of cytoplast-to-cell ratio. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article |
Journal Detail:
|
Title: Letters in applied microbiology Volume: 35 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2002 |
Date Detail:
|
Created Date: 2002-12-03 Completed Date: 2003-02-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 528-32 Citation Subset: IM |
Affiliation:
|
Division of Life Sciences, King's College London, 150 Stamford Street, London SE1 9NN, UK. kamero@kfshrc.edu.sa |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acholeplasma laidlawii
/
growth & development,
physiology* Cell Membrane / metabolism, physiology Fluorescence Fructosediphosphates / metabolism* Glucose / metabolism Glucose-6-Phosphate / metabolism* Liposomes / metabolism* Membrane Fusion / physiology* Oxygen Consumption |
| Chemical | |
Reg. No./Substance:
|
0/Fructosediphosphates; 0/Liposomes; 488-69-7/fructose-1,6-diphosphate; 50-99-7/Glucose; 56-73-5/Glucose-6-Phosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Optimization of a fermentation medium for the production of Penicillin G acylase from Bacillus sp.
Next Document: The effect of starvation stress on the porin protein expression of Escherichia coli in lake water.