| Alternative substrates for triacylglycerol synthesis in isolated adipocytes of different size from the rat. | |
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MedLine Citation:
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PMID: 7030317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolic utilization of 14C-labelled acetate, pyruvate, lactate and glucose by isolated epididymal fat-cells was compared in two groups of rats fed ad libitum, one group young and lean (150-200 g body wt.), the other older and spontaneously obese (500-650 g body wt.). The influence of unlabelled glucose (6 mM) and insulin on substrate utilization by adipocytes was also studied. (1) Pyruvate and lactate were found to be good precursors for fatty-acid synthesis in small fat-cells, but not in larger fat-cells. On the other hand, lactate conversion into CO2 and the glycerol moiety of acylglycerols proceeded activity in both types of cells, and in some cases, it even exceeded the rates of glucose utilization. (2) The addition of glucose or glucose plus insulin, but not insulin alone, enhanced the metabolism of acetate, pyruvate and lactate in both types of fat-cells. (3) Fatty-acid synthesis de novo in large fat-cells was markedly decreased regardless of the substrate utilized. These findings point to lactate as a significant precursor for triacylglycerol synthesis in adipocytes. Furthermore, decreased fatty-acid synthesis de novo appears to be an acquired metabolic deficiency of enlarging adipocytes, independent of precursor substrate availability. |
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Authors:
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A A Francendese; M Digirolamo |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 194 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1981 Feb |
Date Detail:
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Created Date: 1982-01-09 Completed Date: 1982-01-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 377-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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metabolism Adipose Tissue / cytology, drug effects, metabolism* Animals Glucose / metabolism Insulin / pharmacology Lactates / metabolism Male Pyruvates / metabolism Rats Triglycerides / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
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AM-17403/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Lactates; 0/Pyruvates; 0/Triglycerides; 11061-68-0/Insulin; 50-99-7/Glucose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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