Document Detail


Alternative splicing of exon 3b gives rise to ODF2 and Cenexin.
MedLine Citation:
PMID:  18160784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ODF2 was first identified as the major component of the sperm tail outer dense fibers. Additionally, ODF2 is a critical component of the mature centriole of the animal centrosome where it locates to the distal appendages. Moreover, generation of primary cilia strictly depends on ODF2. The mature centriole is characterized further by recruitment of Cenexin. Albeit highly similar in sequence the relationship between ODF2 and Cenexin has not been investigated. We demonstrate here that ODF2 and Cenexin are alternative splice products by identifying a novel exon 3b encoding Cenexin specific amino acids. Even though ODF2 is the main isoform in testicular tissue RT-PCR analyses revealed that isoforms are not restricted to specific tissues.
Authors:
D Hüber; S Hoyer-Fender
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-14
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  119     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-27     Completed Date:  2008-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  68-73     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 S. Karger AG, Basel.
Affiliation:
University of Göttingen, Johann-Friedrich-Blumenbach-Institut für Zoologie, Anthropologie und Entwicklungsbiologie, GZMB, Germany.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing / genetics*
Amino Acid Sequence
Animals
DNA, Complementary / genetics
Exons / genetics*
Gene Expression Regulation
Heat-Shock Proteins / chemistry,  genetics*,  metabolism
Humans
Male
Mice
Molecular Sequence Data
NIH 3T3 Cells
Organ Specificity
Protein Isoforms / chemistry,  genetics,  metabolism
RNA, Messenger / genetics
Sequence Alignment
Testis / metabolism
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Heat-Shock Proteins; 0/Odf2 protein, mouse; 0/Protein Isoforms; 0/RNA, Messenger

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