Document Detail

Alternative spliced variants in the pantetheinase family of genes expressed in human neutrophils.
MedLine Citation:
PMID:  18805469     Owner:  NLM     Status:  MEDLINE    
Pantetheinase (EC is an enzyme that hydrolyzes pantetheine, an intermediate metabolite of coenzyme A, into pantothenic acid (vitamin B(5)) and cysteamine, a potent antioxidant. The pantetheinase gene family consists of three independent genes, pantetheinase/vanin-1/VNN1, GPI-80/VNN2 and vanin-3/VNN3 that are each composed of seven exons. We herein report that human neutrophils express transcripts encoding at least nine splice variants of VNN3 and four splice variants of GPI-80/VNN2. Analysis of the DNA sequence of the human VNN3 gene demonstrated that the VNN3 locus in the human genome as well as the sequence of cDNA clones obtained in this study does not encode the complete VNN3 protein, as previously reported due to a frame shift caused by lack of one nucleotide. Moreover, the VNN3 locus indeed encodes smaller peptides compared to the proteins encoded by the mouse orthologous gene, vanin-3. The anti-GPI-80 monoclonal antibody 3H9 recognized amino acids 120-179 of the GPI-80/VNN2 protein as shown by the results of immunoblotting with recombinant GPI-80/VNN2 variant proteins. Immunoblotting with human neutrophil lysate suggests that the GPI-80/VNN2 variants exist in human neutrophils. The existence of splice variants in the pantetheinase gene family suggests the possibility of alternative roles in addition to canonical enzymatic activity in human neutrophils.
Takeaki Nitto; Teruo Inoue; Koichi Node
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-05
Journal Detail:
Title:  Gene     Volume:  426     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2009-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  IM    
Laboratory of Pharmacotherapy, Yokohama College of Pharmacy, Yokohama, Kanagawa 245-0066, Japan.
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MeSH Terms
Alternative Splicing*
Amidohydrolases / genetics*
Amino Acid Sequence
Cell Adhesion Molecules / genetics
Gene Expression Regulation, Enzymologic*
Hydrolases / genetics
Molecular Sequence Data
Multigene Family*
Neutrophils / metabolism*
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/VNN2 protein, human; EC 3.-/Hydrolases; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/VNN3 protein, human; EC 3.5.1.-/pantetheinase

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