Document Detail


Alternative protein secretion: the Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast.
MedLine Citation:
PMID:  16288715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To examine whether the fission yeast Mam1 ABC transporter can be used for secretion of heterologous proteins, thereby bypassing the classical secretion pathway, we have analyzed chimeric forms of the M-factor precursor. It was demonstrated that GFP can be exported when fused to both the amino-terminal prosequence from mfm1 and a CaaX motif. This secretion was dependent on the Mam1 transporter and not the classical secretion pathway. The secretion efficiency of GFP, however, was relatively low and most of the reporter protein was trapped in the vacuolar membranes. Our findings suggest that the Mam1 ABC protein is a promiscuous peptide transporter that can accommodate globular proteins of a relatively large size. Furthermore, our results help in defining the sequences required for processing and secretion of natural M-factor.
Authors:
Søren Kjaerulff; Sven Müller; Martin Roland Jensen
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Publication Detail:
Type:  Journal Article     Date:  2005-11-02
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  338     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-23     Completed Date:  2006-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1853-9     Citation Subset:  IM    
Affiliation:
Pharmexa A/S, Kogle Allé 6, DK-2970 Hørsholm, Denmark. sku@my.molbio.ku.dk
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / physiology*
Amino Acid Sequence
Green Fluorescent Proteins / secretion*
Molecular Sequence Data
Peptides / secretion*
Protein Precursors / metabolism
Recombinant Fusion Proteins / secretion
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / physiology*,  secretion*
Sequence Alignment
Chemical
Reg. No./Substance:
0/ATP-Binding Cassette Transporters; 0/Peptides; 0/Protein Precursors; 0/Recombinant Fusion Proteins; 0/Schizosaccharomyces pombe Proteins; 0/mam1 protein, S pombe; 0/mfm1 protein, S pombe; 147336-22-9/Green Fluorescent Proteins

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