| Alternative induction of meiotic recombination from single-base lesions of DNA deaminases. | |
| | |
MedLine Citation:
|
PMID: 19237686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Meiotic recombination enhances genetic diversity as well as ensures proper segregation of homologous chromosomes, requiring Spo11-initiated double-strand breaks (DSBs). DNA deaminases act on regions of single-stranded DNA and deaminate cytosine to uracil (dU). In the immunoglobulin locus, this lesion will initiate point mutations, gene conversion, and DNA recombination. To begin to delineate the effect of induced base lesions on meiosis, we analyzed the effect of expressing DNA deaminases (activation-induced deaminase, AID, and APOBEC3C) in germ cells. We show that meiotic dU:dG lesions can partially rescue a spo11Delta phenotype in yeast and worm. In rec12 Schizosaccharomyces pombe, AID expression increased proper chromosome segregation, thereby enhancing spore viability, and induced low-frequency meiotic crossovers. Expression of AID in the germ cells of Caenorhabditis elegans spo-11 induced meiotic RAD-51 foci formation and chromosomal bivalency and segregation, as well as an increase in viability. RNAi experiments showed that this rescue was dependent on uracil DNA-glycosylase (Ung). Furthermore, unlike ionizing radiation-induced spo-11 rescue, AID expression did not induce large numbers of DSBs during the rescue. This suggests that the products of DNA deamination and base excision repair, such as uracil, an abasic site, or a single-stranded nick, are sufficient to initiate and alter meiotic recombination in uni- and multicellular organisms. |
| | |
Authors:
|
Siim Pauklin; Julia S Burkert; Julie Martin; Fekret Osman; Sandra Weller; Simon J Boulton; Matthew C Whitby; Svend K Petersen-Mahrt |
Related Documents
:
|
10080896 - Protein mimicry of dna from crystal structures of the uracil-dna glycosylase inhibitor ... 12627976 - Arabidopsis thaliana ogg1 protein excises 8-hydroxyguanine and 2,6-diamino-4-hydroxy-5-... 11278716 - Base excision and dna binding activities of human alkyladenine dna glycosylase are sens... 7030386 - Nonrandom substitution of 2-aminopurine for adenine during deoxyribonucleic acid synthe... 2416336 - Synthesis and characterization of poly[d(g-z5c)]. b-z transition and inhibition of dna ... 20967616 - Asymmetric overlap extension pcr method for site-directed mutagenesis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-23 |
Journal Detail:
|
Title: Genetics Volume: 182 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-04-30 Completed Date: 2009-07-31 Revised Date: 2010-09-23 |
Medline Journal Info:
|
Nlm Unique ID: 0374636 Medline TA: Genetics Country: United States |
Other Details:
|
Languages: eng Pagination: 41-54 Citation Subset: IM |
Affiliation:
|
INSERM U783, Faculté de Médicine Necker-Enfants Malades, 75730 Paris Cedex 15, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Genetically Modified Apoptosis Caenorhabditis elegans / genetics, metabolism Caenorhabditis elegans Proteins / genetics, metabolism Chromosome Segregation* Cytidine Deaminase / genetics* DNA Breaks, Double-Stranded DNA Repair Esterases / genetics, metabolism Humans In Situ Nick-End Labeling Meiosis / physiology* Rad51 Recombinase / genetics, metabolism Recombination, Genetic* Saccharomyces cerevisiae / genetics, metabolism Saccharomyces cerevisiae Proteins / genetics, metabolism Schizosaccharomyces / genetics, metabolism Schizosaccharomyces pombe Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
//Cancer Research UK |
| Chemical | |
Reg. No./Substance:
|
0/Caenorhabditis elegans Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Schizosaccharomyces pombe Proteins; 0/Spo11 protein; EC 2.7.7.-/Rad51 Recombinase; EC 3.1.-/Esterases; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characteristics and outcomes of revascularized patients with hypertension: an international verapami...
Next Document: Sex-lethal facilitates the transition from germline stem cell to committed daughter cell in the Dros...