| Alternative genotyping method of GSTT1 null/present polymorphism. | |
| | |
MedLine Citation:
|
PMID: 17140374 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Glutathione S-transferase (GST) theta is an enzyme to detoxify xenobiotic compounds. The gene GSTT1 has a null/present polymorphism in one of the targets for cancer susceptibility research. The polymorphism is classified into two types: present type with at least one present allele (heterozygote and homozygote) and null type without a present allele. Although one report showed a method to distinguish the heterozygote from the present homozygote, its use has been limited possibly owing to the difficulty of successful genotyping of long DNA sequences (1460 base pairs). This article reports an alternative method utilizing typical PCR primers specific to the null allele (566 base pairs) and the present allele (458 base pairs). All samples of the GSTT1 null genotype processed by the present PCR method (n = 331), were correctly classified as the null genotype by a conventional method, and the samples of heterozygous (n = 364) or present homozygous (n = 108) genotype as the present genotype; this indicates that it is appropriate for future research to utilize three genotypes of the GSTT1 null/present polymorphism. |
| | |
Authors:
|
Mariko Naito; Yasuyuki Goto; Yoshiko Ishida; Kazuko Nishio; Sayo Kawai; Nobuyuki Hamajima |
Related Documents
:
|
12914384 - Pharmacogenetics of stomach cancer. 10634134 - Analysis of the cyp2d6 gene mutations and their consequences for enzyme function in a w... 11507974 - Genetic polymorphisms of cyp1a1, gstm1 and p53 genes in a unique siberian population of... 11003624 - Molecular diagnosis of a familial nonhemolytic hyperbilirubinemia (gilbert's syndrome) ... 19061604 - Trichoepithelioma. 19502044 - Role of genetic susceptibility to latent adenoviral infection and decreased lung function. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
|
Title: Expert review of molecular diagnostics Volume: 6 ISSN: 1744-8352 ISO Abbreviation: Expert Rev. Mol. Diagn. Publication Date: 2006 Nov |
Date Detail:
|
Created Date: 2006-12-04 Completed Date: 2007-03-13 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 101120777 Medline TA: Expert Rev Mol Diagn Country: England |
Other Details:
|
Languages: eng Pagination: 873-7 Citation Subset: IM |
Affiliation:
|
Department of Preventive Medicine/Biostatistics and Medical Decision Making, 65 Tsurumai-cho Showa-ku, Nagoya 466-8550, Japan. mnaito@med.nagoya-u.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Disease Genetic Testing / methods* Genotype Glutathione Transferase / genetics* Humans Polymorphism, Genetic / genetics* Risk Factors |
| Chemical | |
Reg. No./Substance:
|
EC 2.5.1.-/glutathione S-transferase T1; EC 2.5.1.18/Glutathione Transferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Applications of real-time PCR in the screening of platelet concentrates for bacterial contamination.
Next Document: In vivo optical imaging using quantum dots for the management of brain tumors.