Document Detail


Alternative genotyping method of GSTT1 null/present polymorphism.
MedLine Citation:
PMID:  17140374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glutathione S-transferase (GST) theta is an enzyme to detoxify xenobiotic compounds. The gene GSTT1 has a null/present polymorphism in one of the targets for cancer susceptibility research. The polymorphism is classified into two types: present type with at least one present allele (heterozygote and homozygote) and null type without a present allele. Although one report showed a method to distinguish the heterozygote from the present homozygote, its use has been limited possibly owing to the difficulty of successful genotyping of long DNA sequences (1460 base pairs). This article reports an alternative method utilizing typical PCR primers specific to the null allele (566 base pairs) and the present allele (458 base pairs). All samples of the GSTT1 null genotype processed by the present PCR method (n = 331), were correctly classified as the null genotype by a conventional method, and the samples of heterozygous (n = 364) or present homozygous (n = 108) genotype as the present genotype; this indicates that it is appropriate for future research to utilize three genotypes of the GSTT1 null/present polymorphism.
Authors:
Mariko Naito; Yasuyuki Goto; Yoshiko Ishida; Kazuko Nishio; Sayo Kawai; Nobuyuki Hamajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Expert review of molecular diagnostics     Volume:  6     ISSN:  1744-8352     ISO Abbreviation:  Expert Rev. Mol. Diagn.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-12-04     Completed Date:  2007-03-13     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101120777     Medline TA:  Expert Rev Mol Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  873-7     Citation Subset:  IM    
Affiliation:
Department of Preventive Medicine/Biostatistics and Medical Decision Making, 65 Tsurumai-cho Showa-ku, Nagoya 466-8550, Japan. mnaito@med.nagoya-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Disease
Genetic Testing / methods*
Genotype
Glutathione Transferase / genetics*
Humans
Polymorphism, Genetic / genetics*
Risk Factors
Chemical
Reg. No./Substance:
EC 2.5.1.-/glutathione S-transferase T1; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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