| Alternative bioreactor strategy for probing infection and production. | |
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MedLine Citation:
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PMID: 17951784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production. |
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Authors:
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Yu-Chen Hu; William E Bentley |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 388 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2007-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 419-26 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Baculoviridae / genetics*, growth & development, metabolism Bioreactors* Cell Line Spodoptera / cytology, genetics, metabolism Time Factors beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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