Document Detail


Alternative bioreactor strategy for probing infection and production.
MedLine Citation:
PMID:  17951784     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Probing the baculovirus infection process is essential in optimizing the recombinant protein production. Typically, researchers monitor the infection process in the stirred tank reactor, which, however, contains a population of cells infected at different times after virus inoculation. This chapter describes a two-stage reactor system consisting of an upstream continuous stirred tank reactor and a downstream tubular reactor with segmented plug flow for probing baculovirus infection and production.
Authors:
Yu-Chen Hu; William E Bentley
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  388     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-22     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  419-26     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Baculoviridae / genetics*,  growth & development,  metabolism
Bioreactors*
Cell Line
Spodoptera / cytology,  genetics,  metabolism
Time Factors
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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