Document Detail

Alternative Molecular Tests for Virological Diagnosis.
MedLine Citation:
PMID:  22484997     Owner:  NLM     Status:  Publisher    
Several nucleic acid amplification techniques (NAATs), particularly PCR and real-time PCR, are currently used in the routine clinical laboratories. Such approaches have allowed rapid diagnosis with a high degree of sensitivity and specificity. However, conventional PCR methods have several intrinsic disadvantages such as the requirement for temperature cycling apparatus, and sophisticated and costly analytical equipments. Therefore, amplification at a constant temperature is an attractive alternative method to avoid these requirements. A new generation of isothermal amplification techniques are gaining a wide popularity as diagnostic tools due to their simple operation, rapid reaction and easy detection. The main isothermal methods reviewed here include loop-mediated isothermal amplification, nucleic acid sequence-based amplification, and helicase-dependent amplification. In this review, design criteria, potential of amplification, and application of these alternative molecular tests will be discussed and compared to conventional NAATs.
Francesca Sidoti; Massimiliano Bergallo; Cristina Costa; Rossana Cavallo
Related Documents :
22752097 - Evaluation of filter media for particle number, surface area and mass penetrations.
21091657 - How accurate is in vitro prediction of carcinogenicity?
1941317 - Mechanical characterization of femoral interlocking intramedullary nailing systems.
23219807 - Rapid genotyping of carcinogenic human papillomavirus by loop-mediated isothermal ampli...
6507407 - Late effects of gastric bypass for obesity.
10794087 - Risk of hepatitis and retroviral infections among blood donors and introduction of nucl...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-9
Journal Detail:
Title:  Molecular biotechnology     Volume:  -     ISSN:  1559-0305     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Virology Unit, Department of Public Health and Microbiology, University Hospital San Giovanni Battista di Torino, University of Turin, Via Santena 9, 10126, Turin, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Arousal and Gambling Mode Preference: A Review of the Literature.
Next Document:  Zoonoses and poverty - a long road to the alleviation of suffering.