Document Detail


Altering glomerular epithelial function in vitro using transient and stable transfection.
MedLine Citation:
PMID:  11439746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Damage of the glomerular filtration barrier leads to proteinuria and progressive renal failure. Several independent lines of research have implicated the glomerular epithelial cell (GEC) as a key player in initiation and propagation of pathways leading to glomerulosclerosis. A growing number of molecules activated in this process have been identified. To further define their cellular function, manipulation of these molecules using pharmacological or genetic approaches in tissue culture systems are required. In this study, strategies for altering GEC gene expression by transient and stable transfection of fluorescence labeled proteins will be presented and discussed. The insight gained through these and comparable systems should allow a detailed dissection of the molecular pathways active in GEC function and failure.
Authors:
M Kretzler; V P Teixeira; T Berger; S M Blattner; P G Unschuld; C D Cohen; D Schlöndorff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of nephrology     Volume:  14     ISSN:  1121-8428     ISO Abbreviation:  J. Nephrol.     Publication Date:    2001 May-Jun
Date Detail:
Created Date:  2001-07-06     Completed Date:  2002-06-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9012268     Medline TA:  J Nephrol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  211-9     Citation Subset:  IM    
Affiliation:
Medical Poliklinik, University of Munich, Munich, Germany. kretzler@medpoli.med.uni-muenchen.de
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Humans
Kidney Glomerulus / physiology*
Time Factors
Transfection / methods*
Urothelium / physiology*

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