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Altered transcriptional activity of gene encoding GAPDH in peripheral blood mononuclear cells from patients with cardiac syndrome X - an important part in pathology of microvascular angina?
MedLine Citation:
PMID:  22419929     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Cardiac syndrome X (CSX) is characterized by anginal pain with ECG suggestive of ischaemia and normal coronary arteries at angiography. Pathology of CSX involves microvascular dysfunction and is possibly linked with metabolic syndrome. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. The GAPDH gene is a "housekeeping" gene and is used for normalization in quantitative gene expression assays. The aim of the study was to evaluate GAPDH gene expression in CSX.
MATERIAL AND METHODS: The study was performed in 35 CSX patients and 10 control subjects. mRNA was extracted from peripheral blood mononuclears and the mRNA was assessed by QRT-PCR.
RESULTS: GAPDH gene expression was enhanced in CSX patients vs. controls (93022 ±23837 copies/μg vs. 1067 ±240 copies/μg respectively; p < 0.001). Moreover, transcriptional activity of the GAPDH gene was heterogeneous within the CSX group.
CONCLUSIONS: GAPDH gene expression is markedly enhanced in CSX, which reflects carbohydrate metabolism disturbances and makes the GAPDH gene unsuitable as an endogenous control in patients with CSX.
Authors:
Jozefa Dabek; Jakub Wilczok; Andrzej Kulach; Zbigniew Gasior
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Publication Detail:
Type:  Journal Article     Date:  2010-10-26
Journal Detail:
Title:  Archives of medical science : AMS     Volume:  6     ISSN:  1896-9151     ISO Abbreviation:  Arch Med Sci     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2012-03-15     Completed Date:  2012-10-02     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101258257     Medline TA:  Arch Med Sci     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  709-12     Citation Subset:  -    
Affiliation:
Department of Cardiology, Medical University of Silesia, Katowice, Poland.
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