| Altered sensitivity of deoxyadenosine-resistant mouse leukemia L1210 cells to various kinase inhibitors. | |
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MedLine Citation:
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PMID: 10368649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The deoxyadenosine-resistant mouse leukemia L1210 cell line (Y8) has previously been shown to have phenotypic differences that appear unrelated to the altered properties observed at the level of ribonucleotide reductase (RR). In response to various stress factors, the parental wild-type (WT) L1210 cell line undergoes cell cycle arrest; Y8 cells become apoptotic. These responses are p53-independent. Cell cycle regulation also appears different between the two cell lines, suggesting that Y8 cells are more apoptotic because of alterations in their cell cycle compared to WT cells. In order to study the relationships between cell cycle regulation and apoptosis, the effects of 2-aminopurine (2-AP), wortmannin, and PD98059, were studied on WT and Y8 cells. 2-AP induced G2/M block in both WT and Y8 cells with differences in G0/G1 and S phase contents between the two cell lines. Wortmannin induced G0/G1 block in Y8 cells, while exhibiting no effect on WT cells. PD98059 had no effect on the cell cycle of either WT or Y8 cells. In response to each inhibitor, Y8 cells underwent apoptosis to a much greater extent than the parental WT cell line. These data suggest that the specific pathways that converge on the cell cycle are altered and may be involved in the differences between a tumor cell to block in cell cycle or to undergo apoptosis. |
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Authors:
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L Somerville; J G Cory |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Anticancer research Volume: 19 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 1999 Mar-Apr |
Date Detail:
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Created Date: 1999-07-01 Completed Date: 1999-07-01 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
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Languages: eng Pagination: 1021-6 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, East Carolina University, School of Medicine, Greenville, NC 27858-4354, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Aminopurine
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pharmacology* Androstadienes / pharmacology* Animals Apoptosis / drug effects Deoxyadenosines / pharmacology* Drug Resistance, Neoplasm Flavonoids / pharmacology* G2 Phase / drug effects Leukemia L1210 / drug therapy*, pathology Mice Mitosis / drug effects Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA55540/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Androstadienes; 0/Deoxyadenosines; 0/Flavonoids; 0/PD 98059; 19545-26-7/wortmannin; 452-06-2/2-Aminopurine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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