Document Detail


Altered protein secretion of Chlamydia trachomatis in persistently infected human endocervical epithelial cells.
MedLine Citation:
PMID:  21737500     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chlamydia trachomatis is the most common bacterial infection of the human reproductive tract globally; however, the mechanisms underlying the adaptation of the organism to its natural target cells, human endocervical epithelial cells, are not clearly understood. To secure its intracellular niche, C. trachomatis must modulate the host cellular machinery by secreting virulence factors into the host cytosol to facilitate bacterial growth and survival. Here we used primary human endocervical epithelial cells and HeLa cells infected with C. trachomatis to examine the secretion of bacterial proteins during productive growth and persistent growth induced by ampicillin. Specifically, we observed a decrease in secretable chlamydial protease-like activity factor (CPAF) in the cytosol of host epithelial cells exposed to ampicillin with no evident reduction of CPAF product by C. trachomatis. In contrast, the expression of CopN and Tarp was downregulated, suggesting that C. trachomatis responds to ampicillin exposure by selectively altering the expression of secretable proteins. In addition, we observed a greater accumulation of outer-membrane vesicles from C. trachomatis in persistently infected cells. Taken together, these results suggest that the regulation of both gene expression and the secretion of chlamydial virulence proteins is involved in the adaptation of the bacteria to a persistent infection state in human genital epithelial cells.
Authors:
Jin Wang; Kyla M Frohlich; Lyndsey Buckner; Alison J Quayle; Miao Luo; Xiaogeng Feng; Wandy Beatty; Ziyu Hua; Xiancai Rao; Maria E Lewis; Kelly Sorrells; Kerri Santiago; Guangming Zhong; Li Shen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-07-07
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  157     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-03     Completed Date:  2012-01-23     Revised Date:  2014-05-14    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2759-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Cell Line
Cervix Uteri / cytology,  microbiology
Chlamydia Infections / microbiology*
Chlamydia trachomatis / enzymology,  genetics,  metabolism*
Down-Regulation
Endopeptidases / genetics,  metabolism*
Epithelial Cells / microbiology*
Female
Gene Expression Regulation, Bacterial
Humans
Protein Transport
Uterine Cervical Diseases / microbiology*
Grant Support
ID/Acronym/Agency:
AI055869/AI/NIAID NIH HHS; R01 AI055869/AI/NIAID NIH HHS; R01 AI055869-06/AI/NIAID NIH HHS; R01 AI055869-06S1/AI/NIAID NIH HHS; U19AI061972/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; EC 3.4.-/Endopeptidases; EC 3.4.99.-/CPA factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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