Document Detail


Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome.
MedLine Citation:
PMID:  19903265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The profile of intestinal organic acids in irritable bowel syndrome (IBS) and its correlation with gastrointestinal (GI) symptoms are not clear. We hypothesized in this study that altered GI microbiota contribute to IBS symptoms through increased levels of organic acids. METHODS: Subjects were 26 IBS patients and 26 age- and sex-matched controls. Fecal samples were collected for microbiota analysis using quantitative real-time polymerase chain reaction and culture methods, and the determination of organic acid levels using high-performance liquid chromatography. Abdominal gas was quantified by image analyses of abdominal X-ray films. Subjects completed a questionnaire for GI symptoms, quality of life (QOL) and negative emotion. KEY RESULTS: Irritable bowel syndrome patients showed significantly higher counts of Veillonella (P = 0.046) and Lactobacillus (P = 0.031) than controls. They also expressed significantly higher levels of acetic acid (P = 0.049), propionic acid (P = 0.025) and total organic acids (P = 0.014) than controls. The quantity of bowel gas was not significantly different between controls and IBS patients. Finally, IBS patients with high acetic acid or propionic acid levels presented with significantly worse GI symptoms, QOL and negative emotions than those with low acetic acid or propionic acid levels or controls. CONCLUSIONS & INFERENCES: These results support the hypothesis that both fecal microbiota and organic acids are altered in IBS patients. A combination of Veillonella and Lactobacillus is known to produce acetic and propionic acid. High levels of acetic and propionic acid may associate with abdominal symptoms, impaired QOL and negative emotions in IBS.
Authors:
C Tana; Y Umesaki; A Imaoka; T Handa; M Kanazawa; S Fukudo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-10
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  22     ISSN:  1365-2982     ISO Abbreviation:  Neurogastroenterol. Motil.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-15     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  England    
Other Details:
Languages:  eng     Pagination:  512-9, e114-5     Citation Subset:  IM    
Affiliation:
Department of Behavioral Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / analysis*
Adult
Analysis of Variance
Case-Control Studies
Chromatography, High Pressure Liquid
DNA, Bacterial / genetics
Feces / chemistry*,  microbiology*
Female
Humans
Immunoenzyme Techniques
Irritable Bowel Syndrome / genetics,  microbiology*
Lactobacillus / isolation & purification
Male
Metagenome*
Propionic Acids / analysis*
Quality of Life
Questionnaires
Reverse Transcriptase Polymerase Chain Reaction
Severity of Illness Index
Statistics, Nonparametric
Veillonella / isolation & purification
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Propionic Acids; 64-19-7/Acetic Acid; 79-09-4/propionic acid
Comments/Corrections
Comment In:
Neurogastroenterol Motil. 2010 May;22(5):493-8   [PMID:  20414959 ]
Neurogastroenterol Motil. 2010 Oct;22(10):1147   [PMID:  20840720 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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