Document Detail

Altered phosphorylation as a possible basis for cyclic nucleotide abnormalities in atopic dermatitis.
MedLine Citation:
PMID:  3010615     Owner:  NLM     Status:  MEDLINE    
Exposure of normal mononuclear leukocytes (MNL) to histamine causes heterologous desensitization accompanied by beta adrenergic receptor alterations and increased cyclic AMP-specific phosphodiesterase (PDE) activity. We have demonstrated similar abnormalities in MNL from patients with atopic dermatitis (AD). Considering that these dual changes might be due to altered phosphorylation, we have studied cyclic AMP-dependent protein kinase (PK-A) in untreated and histamine-treated normal MNL and in AD cells. We found that basal or endogenous phosphorylative activity was two-fold higher in preparations from patients with AD. The activity ratios of protein kinase increased significantly in normal MNL after histamine exposure. In contrast, cells from patients with AD failed to show this increase. These findings correlate with similarly increased PDE activity after histamine stimulation of normal, but not AD cells. The increased PK-A activity in both atopic and histamine-desensitized MNL correlates with membrane receptor changes and elevated PDE activity. This enchaned phosphorylation may account for the varied physiological and immunological abnormalities that have been described in AD.
J M Hanifin; S C Chan; C A Hirschman
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta dermato-venereologica. Supplementum     Volume:  120     ISSN:  0365-8341     ISO Abbreviation:  Acta Derm Venereol Suppl (Stockh)     Publication Date:  1985  
Date Detail:
Created Date:  1986-06-10     Completed Date:  1986-06-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370311     Medline TA:  Acta Derm Venereol Suppl (Stockh)     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  47-9     Citation Subset:  IM    
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MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
Dermatitis, Atopic / blood,  metabolism*
Histamine / pharmacology
Monocytes / drug effects,  metabolism*
Protein Kinases / metabolism*
Stimulation, Chemical
Reg. No./Substance:
51-45-6/Histamine; EC 2.7.-/Protein Kinases; EC',5'-Cyclic-AMP Phosphodiesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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