Document Detail

Altered pattern of Na,K-ATPase activity and mRNA during chronic alcohol consumption by juvenile and adolescent rats.
MedLine Citation:
PMID:  18629626     Owner:  NLM     Status:  MEDLINE    
The effect of chronic ethanol consumption on cerebral cortical activity of Na,K-ATPase was determined in Long-Evans (LE) rats fed an ethanol-containing diet beginning at different stages of development. Na,K-ATPase activity was operationally resolved into alpha1 and alpha2/3 isozyme activities. There was no significant difference in Na,K-ATPase activities before and after alcohol consumption in the preparations from adult rats. However, for rats beginning alcohol consumption as adolescents, the alpha2/3 activity was significantly elevated following chronic alcohol consumption. Both LE and Sprague-Dawley rats showed this same selective increase in cortical alpha2/3 activity when rats began alcohol consumption as juveniles. The shift in cortical alpha2/3 activity was not observed in cerebellum or subcortical forebrain and was reversible when rats were fed ethanol throughout the normal adolescent period and then withdrawn and tested 2 weeks later (during the adult period). Levels of isoform-specific mRNA were determined in preparations of cerebral cortices of rats showing elevated alpha2/3 isozyme activities. In these preparations, isoform specific alpha2 and alpha3 mRNA was significantly elevated. There was no effect of ethanol feeding on cortical alpha1 mRNA. These findings indicate that the longer term effects of ethanol on the developing brain include elevated Na,K-ATPase activity and a mechanism that is pre-translational and isoform specific.
Jian Wang; Chun-Shiang Chung; Dennis E Rhoads
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-07-16
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  29     ISSN:  1573-6830     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-03-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  69-80     Citation Subset:  IM    
Department of Biochemistry, University of Rhode Island, Kingston, RI 02881, USA.
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MeSH Terms
Aging / blood
Alcohol Drinking / blood,  genetics*,  metabolism*
Blotting, Northern
Brain / enzymology
Ethanol / blood
Gene Expression Regulation, Enzymologic*
Isoenzymes / genetics,  metabolism
Models, Animal
RNA, Messenger / genetics,  metabolism
Rats, Long-Evans
Rats, Sprague-Dawley
Sodium-Potassium-Exchanging ATPase / genetics*,  metabolism*
Grant Support
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; 64-17-5/Ethanol; EC ATPase

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