Document Detail


Altered morphology and distribution of cellular junction proteins in non-lesional psoriatic epidermis: an insight into disease severity.
MedLine Citation:
PMID:  16314184     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Psoriasis affects 2.7% of the world's population. Keratinocyte proliferation outside the basal layer suggests alterations in cell-cell interactions in affected epidermis. Anomalous expression of proteins forming intercellular junctions has been reported in lesional skin of psoriatic patients. In contrast, little is known about possible alterations in psoriatic non-lesional skin. METHODS: Ten clinically diagnosed psoriasis vulgaris patients and ten controls were studied. All patients were diagnosed with active but controlled psoriatic plates (PASI 3 to 5) and had not received any systemic treatment. The mean age was 43 years for patients and 43.5 years for controls. Four-mm2 skin samples were taken from lesional and non-lesional zones in patients and from abdomen in controls. Five-mum sections were examined for integrity and structural organization by fluorescent labeling of actin filaments and nuclei. Specific antibodies were utilized to localize occludin, E-cadherin, beta-catenin, and proliferation-specific keratins in sections and epidermal sheets. Samples were also processed for immunoblotting with occludin antibody. RESULTS: Lesional and non-lesional psoriatic epidermis from all patients showed keratinocyte hyperproliferation, lack of rete ridges and dermal papillae in the dermal-epidermal junction in some areas. Proteins forming tight and adherens junctions in non-lesional skin keratinocytes from two patients who during the course of the study evolved to uncontrolled disease, showed similar alterations to those observed in lesional skin of all the patients. However, the occludin isoforms expressed were apparently the same in all samples. CONCLUSIONS: Analysis of non-lesional skin in psoriatic patients diagnosed with controlled disease may provide clues about incipient structural abnormalities in the pathogenesis of psoriasis, providing an early diagnostic indicator for evolution to a generalized form of the disease.
Authors:
Alicia Lemini-López; Leopoldo Flores-Romo; Alfredo Arévalo-López; Isaura Meza
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of medical research     Volume:  37     ISSN:  0188-4409     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-11-29     Completed Date:  2006-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  36-44     Citation Subset:  IM    
Affiliation:
Department of Molecular Biomedicine, CINVESTAV-IPN, Mexico, D.F., Mexico.
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*,  pathology
Adult
Aged
Epidermis / metabolism*,  pathology
Female
Humans
Immunohistochemistry
Keratinocytes / metabolism*,  pathology
Male
Membrane Proteins / metabolism*
Middle Aged
Psoriasis / metabolism*,  pathology
Tight Junctions / metabolism*,  pathology
Chemical
Reg. No./Substance:
0/Membrane Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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