Document Detail


Altered levels of primary antioxidant enzymes in progeria skin fibroblasts.
MedLine Citation:
PMID:  10092527     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Free radicals are involved in the aging process. In this study, the profile of primary antioxidant enzymes that scavenge reactive oxygen species (ROS) was examined for the first time in human skin fibroblasts from progeria, a premature aging disease. Altered levels of antioxidant enzymes were found in progeria cells. Basal levels of MnSOD were decreased in progeria cells as well as a blunted induction in response to chronic stress. This change may contribute to the accelerated aging process in progeria cells. In contrast, the levels of CuZnSOD showed no progeria-related change. Two H2O2 removing enzymes demonstrated a significant reduction in progeria cells: only 50% of normal CAT activity and 30% of normal GPX activity can be detected in progeria cells. This diminished H2O2 removing capacity in progeria cells may lead to an imbalance of intracellular ROS and therefore may play an important role in the development of progeria.
Authors:
T Yan; S Li; X Jiang; L W Oberley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  257     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-11     Completed Date:  1999-05-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  163-7     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
B180 Medical Laboratories, The University of Iowa, Iowa City, Iowa, 52242, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Aging
Antioxidants / metabolism*
Blotting, Western
Catalase / metabolism
Cell Line
Child
Enzyme Induction
Fibroblasts / enzymology,  metabolism
Glutathione Peroxidase / metabolism
Humans
Hydrogen Peroxide / metabolism
Male
Matched-Pair Analysis
Peroxidases / metabolism*
Progeria / enzymology*,  metabolism
Reactive Oxygen Species / metabolism
Skin / cytology,  enzymology*
Stress, Physiological
Superoxide Dismutase / metabolism*
Grant Support
ID/Acronym/Agency:
P01-CA 66081/CA/NCI NIH HHS; P50-DE10758/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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