| Altered ion transporter expression in bronchial epithelium in mountaineers with high-altitude pulmonary edema. | |
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MedLine Citation:
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PMID: 14555664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypoxia inhibits activity and expression of transport proteins of cultured lung alveolar epithelial cells. Here we tested whether hypoxia at high altitude affected the expression of ion transport proteins in tissues obtained from controls and mountaineers with high-altitude pulmonary edema (HAPE) at the Capanna Margherita (4,559 m). Expression was determined by RT-PCR and Western blots from brush biopsies of bronchial epithelium and from leukocytes obtained before and during the stay at high altitude. At low altitude, amounts of mRNAs were not different between control and HAPE-susceptible subjects. At high altitude, the amount of mRNA of Na-K-ATPase, CFTR, and beta-actin of brush biopsies did not change in controls but decreased significantly (-60%) in HAPE-susceptible subjects. There was no change in Na channel mRNAs at high altitude in controls and HAPE. No statistically significant correlation was found between the expression of Na transporters and PO2 and O2 saturation. In leukocytes, 28S-rRNA and Na-K-ATPase decreased at altitude in control and HAPE-susceptible subjects, but no significant change in Na-K-ATPase protein was found. Hypoxia-inducible factor-1alpha mRNA and GAPDH mRNA tended to increase in leukocytes obtained from HAPE-susceptible subjects at high altitude but did not change in controls. These results show that hypoxia induces differences in mRNA expression of ion transport-related proteins between HAPE-susceptible and control subjects but that these changes may not necessarily predict differences in protein concentration or activity. It is therefore unclear whether these differences are related to the pathophysiology of HAPE. |
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Authors:
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Heimo Mairbäurl; Florian Schwöbel; Sabine Höschele; Marco Maggiorini; Simon Gibbs; Erik R Swenson; Peter Bärtsch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 95 ISSN: 8750-7587 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2003 Nov |
Date Detail:
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Created Date: 2003-10-13 Completed Date: 2004-06-15 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 1843-50 Citation Subset: IM |
Affiliation:
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Division of Sports Medicine, Department of Medicine, University of Heidelberg, Germany. heimo.mairbaeurl@med.uni-heidelberg.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Altitude Sickness / physiopathology* Bronchi / physiology Carbon Dioxide / blood Cystic Fibrosis Transmembrane Conductance Regulator / genetics* Female Gene Expression / physiology Humans Leukocytes / physiology Male Middle Aged Mountaineering / physiology* Oxygen / blood Pulmonary Edema / physiopathology* RNA, Messenger / analysis Respiratory Mucosa / physiology Sodium-Potassium-Exchanging ATPase / genetics* |
| Chemical | |
Reg. No./Substance:
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0/CFTR protein, human; 0/RNA, Messenger; 124-38-9/Carbon Dioxide; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 7782-44-7/Oxygen; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
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