Document Detail


Altered immune phenotype in peripheral blood cells of patients with scleroderma-associated pulmonary hypertension.
MedLine Citation:
PMID:  20973920     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pulmonary arterial hypertension is a common and fatal complication of scleroderma that may involve inflammatory and autoimmune mechanisms. Alterations in the gene expression of peripheral blood mononuclear cells have been previously described in patients with pulmonary arterial hypertension. Our goal is to identify differentially expressed genes in peripheral blood mononuclear cells in scleroderma patients with and without pulmonary hypertension as biomarkers of disease. Gene expression analysis was performed on a Microarray Cohort of scleroderma patients with (n = 10) and without (n = 10) pulmonary hypertension. Differentially expressed genes were confirmed in the Microarray Cohort and validated in a Validation Cohort of scleroderma patients with (n = 15) and without (n = 19) pulmonary hypertension by RT-qPCR. We identified inflammatory and immune-related genes including interleukin-7 receptor (IL-7R) and chemokine receptor 7 as differentially expressed in patients with scleroderma-associated pulmonary hypertension. Flow cytometry confirmed decreased expression of IL-7R on circulating CD4+ T-cells from scleroderma patients with pulmonary hypertension. Differences exist in the expression of inflammatory and immune-related genes in peripheral blood cells from patients with scleroderma-related pulmonary hypertension compared to those with normal pulmonary artery pressures. These findings may have implications as biomarkers to screen at-risk populations for early diagnosis and provide insight into mechanisms of scleroderma-related pulmonary hypertension.
Authors:
Michael G Risbano; Christina A Meadows; Christopher D Coldren; Tiffany J Jenkins; Michael G Edwards; David Collier; Wendy Huber; Douglas G Mack; Andrew P Fontenot; Mark W Geraci; Todd M Bull
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and translational science     Volume:  3     ISSN:  1752-8062     ISO Abbreviation:  Clin Transl Sci     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2011-06-16     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101474067     Medline TA:  Clin Transl Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  210-8     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley Periodicals, Inc.
Affiliation:
Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver, Aurora, USA. onabsir@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Blood Cells / immunology*
CD4-Positive T-Lymphocytes / immunology
Cluster Analysis
Cohort Studies
Demography
Female
Flow Cytometry
Hemodynamics
Humans
Hypertension, Pulmonary / blood,  etiology,  genetics,  immunology
Male
Middle Aged
Oligonucleotide Array Sequence Analysis
Phenotype
Receptors, Interleukin-7 / immunology
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Scleroderma, Systemic / blood*,  complications*,  genetics,  immunology
Grant Support
ID/Acronym/Agency:
K08 HLO72858-01A2//PHS HHS
Chemical
Reg. No./Substance:
0/Receptors, Interleukin-7
Comments/Corrections
Comment In:
Clin Transl Sci. 2011 Feb;4(1):72   [PMID:  21348960 ]
Erratum In:
Clin Transl Sci. 2010 Dec;3(6):340
Note: Hunt, James [added]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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