| Altered guanylyl-cyclase activity in vitro of pulmonary arteries from fetal lambs with congenital diaphragmatic hernia. | |
| | |
MedLine Citation:
|
PMID: 12091244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Nitric oxide (NO) plays a major role in the modulation of perinatal pulmonary vascular tone. Congenital diaphragmatic hernia (CDH), a major cause of severe persistent pulmonary hypertension of the newborn (PPHN), is often refractory to inhaled NO. Alterations in NO/cyclic guanosine 3',5' monophosphate (cGMP)-mediated pulmonary vasodilatation may contribute to PPHN in CDH. We assessed NO/cGMP-mediated pulmonary vasorelaxation in vitro in 140-d gestational lamb fetuses with surgically created left CDH (term = 147 d) to age-matched controls. Relaxation of fourth generation intralobar pulmonary artery rings in response to the endothelium-dependent vasodilator, acetylcholine (ACh), and to the specific inhibitor of cGMP-phosphodiesterase (PDE), zaprinast, did not differ between the two groups. By contrast, relaxation in response to the calcium ionophore A23187 was impaired in CDH as compared with control animals. Relaxation in response to the NO donor sodium nitroprusside (SNP) (a direct activator of soluble guanylyl cyclase [sGC]) was also impaired in CDH animals as compared with controls. Repeating the challenge increased vasorelaxation in response to SNP in CDH as compared with control animals. Immunohistochemistry revealed the presence of endothelial NO-synthase in the endothelium of pulmonary arteries from both control and CDH animals. We conclude that endothelium-dependent vasodilatation in response to ACh and A23187 was differently affected in the fetal surgical CDH-lamb model. Furthermore, activity of sGC but not that of PDE was impaired in CDH animals. PPHN and decreased inhaled NO responsiveness in CDH may involve decreased sGC activity. |
| | |
Authors:
|
Bernard Thébaud; Thierry Petit; Pascal De Lagausie; Josette Dall'Ava-Santucci; Jean-Christophe Mercier; A Tuan Dinh-Xuan |
Related Documents
:
|
19231384 - Differential effects of natriuretic peptides on arterial and venous coronary artery byp... 7527044 - Effect of sympathetic denervation on the relaxing responses of rabbit arterial smooth m... 1717794 - Endothelial function of human gastroepiploic artery. implications for its use as a bypa... 2820194 - Comparative effects of some calcium-channel blockers on human peripheral arteries and v... 15980954 - Asymptomatic renal infarction, due to fibromuscular dysplasia, in a young woman with 11... 15506314 - Endovascular treatment of arteriovenous malformation in a child. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: American journal of respiratory cell and molecular biology Volume: 27 ISSN: 1044-1549 ISO Abbreviation: Am. J. Respir. Cell Mol. Biol. Publication Date: 2002 Jul |
Date Detail:
|
Created Date: 2002-07-01 Completed Date: 2002-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 42-7 Citation Subset: IM |
Affiliation:
|
Service de Physiologie-Explorations Fonctionnelles, CHU Cochin, Assistance Publique-Hôpitaux de Paris, Université Paris V, France. bthebaud@ualberta.ca |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylcholine
/
pharmacology Animals Animals, Newborn Calcimycin / pharmacology Cyclic GMP / metabolism Disease Models, Animal Epithelium / drug effects, metabolism, physiology Female Fetus / enzymology, surgery Guanylate Cyclase Hernia, Diaphragmatic / enzymology* Hypertension, Pulmonary / enzymology Immunohistochemistry Ionophores / pharmacology Isometric Contraction / physiology Nitric Oxide / metabolism Nitric Oxide Synthase / analysis, metabolism* Nitric Oxide Synthase Type III Nitroprusside / pharmacology Pulmonary Artery / drug effects, enzymology*, metabolism Receptors, Cytoplasmic and Nuclear / metabolism* Sheep Vasodilation / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Ionophores; 0/Receptors, Cytoplasmic and Nuclear; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; 51-84-3/Acetylcholine; 52665-69-7/Calcimycin; 7665-99-8/Cyclic GMP; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 4.6.1.2/Guanylate Cyclase; EC 4.6.1.2/soluble guanylyl cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Interaction of alveolar macrophages and airway epithelial cells following exposure to particulate ma...
Next Document: Identification of a novel IL-6 isoform binding to the endogenous IL-6 receptor.