Document Detail

Altered gene expression associated with apoptosis in a pre-B-leukemic cell line following cross-linking of MHC class I.
MedLine Citation:
PMID:  9056426     Owner:  NLM     Status:  MEDLINE    
The major histocompatibility complex class I (MHC-I) has recently been shown not only to present antigens to the immune system but also to mediate transmembrane signaling, resulting in activation, inactivation, or apoptosis. Such signaling has been observed in both normal and malignant cells of the B and T cell lineage. Cross-linking of MHC-I on the pre-B-acute-lymphocytic cell line KM-3 induces an apoptotic process, which becomes evident after approximately 12 h. In order to better understand the mechanisms regulating this apoptotic process, we have investigated both gene expression and the effect of cross-linking on certain intracellular events. Differential display PCR was used to isolate two gene fragments whose level of expression was associated with the induction of apoptosis as they were downregulated in KM-3 cells following MHC-I cross-linking. These genes encode novel molecules whose function remains to be elucidated. It was further demonstrated that the apoptotic process was not accompanied by changes in [Ca2+]i, the level of activation of NF-kappaB, or changes in protein kinase C activity and that the initiation of apoptosis could be prevented by phorbol ester treatment. It is thus suggested that multiple, fine-tuned molecular events determine the outcome of cross-linking of MHC-I in this pre-B-lymphocytic cell line.
N Nilsson; M Wallén-Ohman; M Ohlin; C A Borrebaeck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  231     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  190-7     Citation Subset:  IM    
Department of Immunotechnology, Lund University, Lund, S-220 07, Sweden.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U77669;  U77670
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MeSH Terms
Amino Acid Sequence
Base Sequence
Calcium / metabolism
DNA Fragmentation
Gene Expression Regulation, Leukemic*
Histocompatibility Antigens Class I / metabolism*
Molecular Sequence Data
NF-kappa B / metabolism
Polymerase Chain Reaction
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*,  metabolism,  pathology
Protein Kinase C / metabolism
RNA Probes
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
Reg. No./Substance:
0/Histocompatibility Antigens Class I; 0/NF-kappa B; 0/RNA Probes; 16561-29-8/Tetradecanoylphorbol Acetate; 7440-70-2/Calcium; EC Kinase C

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