| Altered expression of type II sodium/phosphate cotransporter in polycystic kidney disease. | |
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MedLine Citation:
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PMID: 11004225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Renal phosphate (Pi) absorption is mediated via the type II sodium/Pi cotransporter (NaPi-2) in the brush border membrane (BBM) of proximal tubules. Simultaneous detection of NaPi-2 mRNA by in situ hybridization and of NaPi-2 immunoreactivity by immunohistochemistry was performed to investigate the distribution of the cotransporter in healthy control rats and during progression of autosomal dominant polycystic kidney disease (ADPKD). The purpose of the study was to disclose a relation between proximal tubular cell differentiation and NaPi-2 expression. In controls, NaPi-2 expression was present in the entire proximal tubule. In the Han:SPRD (cy/+) model for ADPKD, the proximal nephron is primarily affected by the cystic changes. Epithelial proliferation and impaired epithelial-matrix interaction result in a loss of cell differentiation that eventually leads to cystic enlargement of the nephron. Normal expression of NaPi-2 in this model was found only in tubules with intact BBM. Loss of BBM and cellular interdigitation were paralleled by the loss of NaPi-2 in situ hybridization and immunoreactive signals. These changes were moderate and focal in 2-mo-old rats and generalized all over the cortex after 8 mo. Advanced renal damage in the older PKD group was associated with mild phosphaturia, which suggests functional insufficiency of tubular NaPi-2 reabsorption. These data show how proliferative changes and loss of tubular epithelial differentiation in ADPKD may prevent functional expression of the NaPi-2 system in the proximal tubule in a rapidly progressive manner. NaPi-2 in proximal tubule BBM is suggested to play an important role in impaired tubular absorption of Pi in renal disease. |
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Authors:
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M Vogel; B Kränzlin; J Biber; H Murer; N Gretz; S Bachmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Society of Nephrology : JASN Volume: 11 ISSN: 1046-6673 ISO Abbreviation: J. Am. Soc. Nephrol. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-10-10 Completed Date: 2000-10-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9013836 Medline TA: J Am Soc Nephrol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1926-32 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Medical Research Center, Klinikum Mannheim, University of Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / genetics, metabolism* Immunohistochemistry In Situ Hybridization Kidney Tubules, Proximal / metabolism, pathology Male Microvilli / metabolism Nephrons / pathology Polycystic Kidney, Autosomal Dominant / metabolism*, pathology RNA, Messenger / metabolism Rats Rats, Inbred Strains Sodium-Phosphate Cotransporter Proteins Sodium-Phosphate Cotransporter Proteins, Type II Symporters* |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/RNA, Messenger; 0/Sodium-Phosphate Cotransporter Proteins; 0/Sodium-Phosphate Cotransporter Proteins, Type II; 0/Symporters |
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