Document Detail


Altered expression of selected microRNAs in melanoma: antiproliferative and proapoptotic activity of miRNA-155.
MedLine Citation:
PMID:  19578755     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Altered expression of microRNAs (miRNAs) has been detected in cancer, suggesting that these small non-coding RNAs can act as oncogenes or tumor suppressor genes. In the present study, we investigated the expression of miRNA-17-5p, miRNA-18a, miRNA-20a, miRNA-92a, miRNA-146a, miRNA-146b and miRNA-155 by real-time quantitative RT-PCR in a panel of melanocyte cultures and melanoma cell lines and explored the possible role of miRNA-155 in melanoma cell proliferation and survival. The analyzed miRNAs were selected on the basis of previous studies strongly supporting their involvement in cancer development and/or progression. We found that miRNA-17-5p, miRNA-18a, miRNA-20a, and miRNA-92a were overexpressed, whereas miRNA-146a, miRNA-146b and miRNA-155 were down-regulated in the majority of melanoma cell lines with respect to melanocytes. Ectopic expression of miRNA-155 significantly inhibited proliferation in 12 of 13 melanoma cell lines with reduced levels of this miRNA and induced apoptosis in 4 out of 4 cell lines analyzed. In conclusion, our data further support the finding of altered miRNA expression in melanoma cells and establish for the first time that miRNA-155 is a negative regulator of melanoma cell proliferation and survival.
Authors:
Lauretta Levati; Ester Alvino; Elena Pagani; Diego Arcelli; Patrizia Caporaso; Sergio Bondanza; Gianpiero Di Leva; Manuela Ferracin; Stefano Volinia; Enzo Bonmassar; Carlo Maria Croce; Stefania D'Atri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of oncology     Volume:  35     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-06     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  393-400     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Oncology, Istituto Dermopatico dell'Immacolata-IRCCS, I-00167 Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Cell Line, Tumor
Cell Proliferation
Humans
Melanoma / genetics*,  pathology*
MicroRNAs / genetics*,  physiology
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/MIRN146 microRNA, human; 0/MIRN155 microRNA, human; 0/MIRN16 microRNA, human; 0/MIRN17 microRNA, human; 0/MicroRNAs

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