| Altered expression of rat renal cortical OAT1 and OAT3 in response to bilateral ureteral obstruction. | |
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MedLine Citation:
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PMID: 16316345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Bilateral ureteral obstruction (BUO) is characterized by the development of hemodynamic and tubular lesions. However, little is known about the expression of organic anion renal transporters. The objective of this work was to study the renal excretion of p-aminohippurate (PAH) and the cortical renal expression of the organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) in BUO rats. METHODS: Male Wistar rats underwent bilateral obstruction of the proximal ureters for 24 hours (BUO) or sham operation. After 24 hours of ureteral releasing, the following studies were performed: PAH renal excretion employing conventional clearance techniques and OAT1 and OAT3 abundance (homogenates, intracellular and basolateral plasma membrane fractions from renal cortex) using immunoblotting and immunocytochemical techniques (light microscopic and confocal immunofluorescence microscopic analysis). RESULTS: BUO rats showed a lower renal excretion of PAH. In obstructed kidneys, immunoblotting revealed a significant decrease in the abundance of both OAT1 and OAT3 in basolateral plasma membranes from renal cortex. An increase of OAT1 expression was observed in homogenates and in intracellular membrane fractions in kidneys from BUO rats compared with sham-operated ones, indicating an internalization of this carrier. Immunocytochemical techniques confirmed these results. On the contrary, OAT3 expression was reduced both in homogenates and in intracellular membrane fractions in obstructed kidneys. CONCLUSION: BUO was associated with down-regulation of OAT1 and OAT3 in basolateral plasma membranes from proximal tubule cells, thus these carriers may play important roles in the impaired organic anion excretion displayed in the obstructed kidney. |
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Authors:
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Silvina R Villar; Anabel Brandoni; Naohiko Anzai; Hitoshi Endou; Adriana M Torres |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Kidney international Volume: 68 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-30 Completed Date: 2006-02-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: United States |
Other Details:
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Languages: eng Pagination: 2704-13 Citation Subset: IM |
Affiliation:
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Farmacolog?a, Facultad de Ciencias Bioqu?micas y Farmac?uticas, Universidad Nacional de Rosario, Conicet, Argentina. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure Body Weight Cell Membrane / metabolism Down-Regulation Fluorescent Antibody Technique Glomerular Filtration Rate Intracellular Membranes / metabolism Kidney Cortex / metabolism* Male Organic Anion Transport Protein 1 / metabolism* Organic Anion Transporters, Sodium-Independent / metabolism* Potassium / blood Rats Rats, Wistar Sodium / blood Ureteral Obstruction / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Organic Anion Transport Protein 1; 0/Organic Anion Transporters, Sodium-Independent; 0/Slc22a6 protein, rat; 0/organic anion transport protein 3; 7440-09-7/Potassium; 7440-23-5/Sodium |
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