Document Detail

Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet.
MedLine Citation:
PMID:  21699519     Owner:  NLM     Status:  In-Data-Review    
Background  Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime. Objectives  To investigate the changes of epidermal lipid properties after long-term UV radiation with SED. Materials and methods  Hairless mice were irradiated three times weekly for 15 weeks at an SED of UV (UVB: 20 mJ/cm(2) ; UVA: 14 J/cm(2) ). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times. Results  The SED UV-irradiated group showed increased TEWL after 12 weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide. Conclusions  Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.
Hana Bak; Seung-Phil Hong; Se-Kyoo Jeong; Eung-Ho Choi; Sang E Lee; Seung-Hun Lee; Sung-Ku Ahn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of dermatology     Volume:  50     ISSN:  1365-4632     ISO Abbreviation:  Int. J. Dermatol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243704     Medline TA:  Int J Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  832-7     Citation Subset:  IM    
Copyright Information:
© 2011 The International Society of Dermatology.
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea, Department of Dermatology and Institute of Basic Medical Science, Yonsei University Wonju College of Medicine, Wonju, Korea, NeoPharm Co. Ltd, Daejeon, Korea, and Department of Dermatology, Yonsei University College of Medicine, Gangnam Severance Hospital, Seoul, Korea.
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