Document Detail


Altered eicosanoid biosynthesis in selenium-deficient endothelial cells.
MedLine Citation:
PMID:  10699749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Selenium (Se) is an integral part of the Se-dependent glutathione peroxidase (Se-GSH-Px) catalytic domain. By modulating the cellular levels of fatty acid hydroperoxides, Se-GSH-Px can influence key enzymes of arachidonic acid cascade, in this case cyclooxygenase (COX) and lipoxygenase (LOX). To investigate this phenomenon, the effects of cellular Se status on the enzymatic oxidation of arachidonic acid were investigated in bovine mammary endothelial cells (BMEC), which were cultured in either Se-deficient (-Se) or Se-adequate (+Se) media. When stimulated with calcium ionophore A23187, BMEC produced eicosanoids of both COX and LOX pathways. Compared with the Se-adequate cells, the production of prostaglandin I(2) (PGI(2)), prostaglandin F(2) (PGF(2alpha)), and prostaglandin E(2) (PGE(2)) was significantly decreased in Se-deficient cells, whereas the production of thromboxane A(2) (TXA(2)) was markedly increased in the -Se BMEC cultures. Although the enzymatic oxidation of arachidonic acid by the LOX pathway was found to be relatively less than by the COX pathway, the BMEC cultured in -Se media produced significantly more 15-hydroperoxyeicosatetraenoic acid (15-HPETE) than the +Se cells produced. Based on these results, we postulate that cellular Se status plays an important regulatory role in the enzymatic oxidation of arachidonic acid by the COX and LOX pathways. The altered eicosanoid biosynthesis, especially the overproduction of 15-HPETE, in -Se BMEC may be one of the underlying biochemical phenomena responsible for vascular dysfunction during Se deficiency.
Authors:
Y Z Cao; C C Reddy; L M Sordillo
Related Documents :
4514999 - Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis.
7153109 - Effects of arachidonic acid, pgf2 alpha, and a pgh2 analogue on airway diameters in dogs.
9131679 - Eicosanoids and fat emulsions in acute respiratory distress syndrome patients.
3109689 - Evidence for increased activity of mouse brain fatty acid cyclooxygenase following drug...
3125839 - Examination of mouse and rat tissues for evidence of dual forms of the fatty acid cyclo...
8295899 - The metabolism of arachidonic acid to prostaglandin e2 in plerocercoids of spirometra e...
2574569 - Inhibition of biliary cholesterol and phospholipid secretion during cyclobutyrol-induce...
12162339 - Development of the indicator amino acid oxidation technique in chickens: calibration of...
24242059 - Use of water and edta extractions to estimate available (free and reversibly bound) phe...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  28     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-04-11     Completed Date:  2000-04-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Affiliation:
Department of Veterinary Science, The Pennsylvania State University, University Park, PA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid / metabolism
Calcimycin / pharmacology
Cattle
Cells, Cultured
Culture Media
Dinoprost / biosynthesis
Dinoprostone / biosynthesis
Eicosanoids / biosynthesis*
Endothelium, Vascular / cytology,  drug effects,  metabolism*
Epoprostenol / biosynthesis
Female
Leukotrienes / biosynthesis
Lipid Peroxides / biosynthesis
Lipoxygenase / metabolism
Mammary Arteries
Oxidation-Reduction
Prostaglandin-Endoperoxide Synthases / metabolism
Selenium / deficiency,  pharmacology*
Thromboxane A2 / biosynthesis
Grant Support
ID/Acronym/Agency:
R01HL60044/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/Eicosanoids; 0/Leukotrienes; 0/Lipid Peroxides; 35121-78-9/Epoprostenol; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 551-11-1/Dinoprost; 57576-52-0/Thromboxane A2; 67675-14-3/15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; 7782-49-2/Selenium; EC 1.13.11.12/Lipoxygenase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Different roles for nitrogen monoxide and peroxynitrite in lipid peroxidation induced by activated n...
Next Document:  Ionizing radiation potentiates the induction of nitric oxide synthase by interferon-gamma (Ifn-gamma...