| Altered dopamine and serotonin metabolism in the dopamine-denervated and serotonin-hyperinnervated neostriatum of adult rat after neonatal 6-hydroxydopamine. | |
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MedLine Citation:
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PMID: 8071864 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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3,4-Dihydroxyphenylalanine (L-DOPA), 5-hydroxy-l-tryptophan (5-HTP), dopamine (DA), 5-hydroxytryptamine (5-HT) and their metabolites were measured in the rostral neostriatum of adult rats neonatally lesioned with 6-hydroxydopamine (6-OHDA) after treatment with NSD-1015 (aromatic L-amino acid decarboxylase inhibitor), pargyline (monoamine oxidase inhibitor) or probenecid (blocker of acidic metabolite efflux). Binding experiments with DA and 5-HT transporter ligands allowed us to relate observed changes to the number of DA and 5-HT terminals. One and 3 months after the lesions were made, DA, 3,4-dihydroxyphenylacetic acid (DOPAC)- and homovanillic acid (HVA)-content as well as [3H]N-[1-(2-benzo(b)-thiophenyl)cyclohexyl]piperidine ([3H]BTCP) binding were lowered to 1% to 2% of control values, reflecting a permanent DA denervation. After 1 month, 5-HT content was increased by 96% and 5-hydroxyindole-3-acetic acid (5-HIAA) was increased by 50% in the presence of control levels of [3H]citalopram binding, suggesting that there was an increased amount of 5-HT per neostriatal 5-HT terminal. At 3 months, 5-HT content had increased by 205%, 5-HIAA remained increased by 50% and [3H]citalopram binding had reached 170% of control values, indicating a persistent increase in 5-HT content within an excessive number of 5-HT terminals (hyperinnervation). At both survival times, neostriatal L-DOPA accumulation after NSD-1015 was low, but ratios of L-DOPA to DA and of L-DOPA to [3H]BTCP were greatly elevated. 5-HTP accumulation was increased, but ratios of 5-HTP to 5-HT were lower than control, despite unchanged ratios of 5-HTP to [3H]citalopram.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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E Molina-Holgado; K M Dewar; L Descarries; T A Reader |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 270 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1994-09-28 Completed Date: 1994-09-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 713-21 Citation Subset: IM |
Affiliation:
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Département de Physiologie, Faculté de Médecine, Université de Montréal, Quebec, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Carrier Proteins / metabolism Denervation Dopamine / metabolism* Dopamine Plasma Membrane Transport Proteins Female Male Membrane Glycoproteins / metabolism Membrane Transport Proteins* Monoamine Oxidase Inhibitors / pharmacology Neostriatum / drug effects, metabolism* Nerve Tissue Proteins* Oxidopamine / pharmacology* Rats Rats, Sprague-Dawley Serotonin / metabolism* Serotonin Plasma Membrane Transport Proteins |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Dopamine Plasma Membrane Transport Proteins; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Monoamine Oxidase Inhibitors; 0/Nerve Tissue Proteins; 0/Serotonin Plasma Membrane Transport Proteins; 0/Slc6a4 protein, rat; 1199-18-4/Oxidopamine; 50-67-9/Serotonin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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