Document Detail

Altered cytoplasmic/nuclear distribution of the c-myc protein in differentiating ML-1 human myeloid leukemia cells.
MedLine Citation:
PMID:  8518229     Owner:  NLM     Status:  MEDLINE    
The c-myc gene is thought to play a role in cell proliferation and differentiation; for example, constitutive expression of an exogenously introduced c-myc gene can inhibit differentiation in hematopoietic cell lines. Expression of the endogenous c-myc gene has now been monitored during the differentiation, and associated loss of proliferation, of ML-1 human myeloblastic leukemia cells: c-myc mRNA remains detectable, at decreased levels, during differentiation along the monocyte/macrophage pathway induced with 12-O-tetradecanoylphorbol-13-acetate. c-myc protein also remains present, at undiminished levels, in mature, nonproliferative cells (assessed by immunoblotting and flow cytometry). The protein is, however, readily detectable in the cytoplasm of 12-O-tetradecanoylphorbol-13-acetate-induced cells, and some of this cytoplasmic c-myc exhibits a shift in electrophoretic mobility compared to the predominantly nuclear c-myc in uninduced cells. Furthermore, although c-myc protein continues to be synthesized in the mature cells (assessed by metabolic labeling/immunoprecipitation), loss of the protein from the cytoplasm and accumulation in the nucleus are slowed (assessed by pulse-chase metabolic labeling). These findings suggest that, during the 12-O-tetradecanoylphorbol-13-acetate-induced differentiation and loss of proliferation of ML-1 cells, c-myc protein is regulated through alterations that affect its cytoplasmic/nuclear distribution rather than its total cellular content.
R W Craig; H L Buchan; C I Civin; M B Kastan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  4     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-07-26     Completed Date:  1993-07-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-57     Citation Subset:  IM    
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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MeSH Terms
Cell Differentiation / drug effects,  physiology
Cell Nucleus / chemistry*
Cytoplasm / chemistry*
Leukemia, Myeloid / metabolism*,  pathology
Nuclear Proteins / analysis*
Proto-Oncogene Proteins c-myc / analysis*
Tetradecanoylphorbol Acetate
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Nuclear Proteins; 0/Proto-Oncogene Proteins c-myc; 16561-29-8/Tetradecanoylphorbol Acetate

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