Document Detail


Altered cytokeratin expression during chemoprevention of experimental hamster buccal pouch carcinogenesis by garlic.
MedLine Citation:
PMID:  11903819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Cytokeratins (also known as keratins (K)) are members of the family of intermediate filaments and form major components of the mammalian epithelial cell cytoskeleton. Cytokeratins have emerged as reliable cellular markers of oral cancer development and chemoprevention because of their abundance, stability and high antigenicity. METHODS: We investigated the effect of aqueous garlic extract on cytokeratin expression during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. Hamsters were divided into four groups of six animals. Animals in group 1 were painted with a 0.5% solution of DMBA in liquid paraffin, on the right buccal pouches, three times a week for 14 weeks. Group 2 animals were painted with DMBA as in group 1 and also received 250 mg/kg body weight aqueous garlic extract orally on alternate days to the DMBA application. Group 3 animals received garlic extract only, as in group 2. Group 4 animals received neither DMBA nor garlic extract and served as the control. The hamsters were killed after an experimental period of 14 weeks. RESULTS: Cytokeratin expression was studied using human monoclonal antibodies AE1 and AE3, which react with type I and II keratins. In DMBA-induced squamous cell carcinomas, decreased expression of high molecular weight keratins was observed. Administration of garlic extract to animals painted with DMBA suppressed HBP carcinomas and restored normal cytokeratin expression. CONCLUSION: The results of the present study suggest that inhibition of HBP carcinogenesis by garlic may be due to its regulatory effects on differentiation, tumour invasiveness, migratory and metastatic potential. We suggest that one of the mechanisms of tumour inhibition by garlic is an influence on cellular differentiation.
Authors:
S Balasenthil; K S Rao; S Nagini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  31     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-03-20     Completed Date:  2002-08-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  142-6     Citation Subset:  D; IM    
Affiliation:
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar-608,002, Tamil Nadu, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal
Antineoplastic Agents, Phytogenic / administration & dosage*
Blotting, Western
Carcinoma, Squamous Cell / chemically induced,  metabolism*,  prevention & control*
Cell Differentiation / drug effects
Cheek
Chemoprevention
Cricetinae
Electrophoresis, Polyacrylamide Gel
Garlic*
Humans
Keratins / biosynthesis*
Male
Mesocricetus
Mouth Neoplasms / chemically induced,  metabolism*,  prevention & control*
Phytotherapy*
Random Allocation
Sulfinic Acids / administration & dosage*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antineoplastic Agents, Phytogenic; 0/Sulfinic Acids; 539-86-6/allicin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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