| Altered control of gastric acid secretion in gastrin-cholecystokinin double mutant mice. | |
| | |
MedLine Citation:
|
PMID: 14762785 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND & AIMS: Three pathways control gastric acid secretion: the gastrin-enterochromaffin-like (ECL) cell axis, the vagus-parietal cell axis, and the cholecystokinin (CCK)-D cell axis. Mice lacking gastrin or both gastrin and CCK were examined to determine the role of the hormones. METHODS: Acid was measured after pylorus ligation, and biopsies from gastrin knockout (KO), gastrin-CCK double-KO, and wild-type (WT) mice were collected for biochemical, immunocytochemical, and electron-microscopic examination. RESULTS: The ECL cells were inactive in both groups of mutant mice but the cell number was unaffected. Both parietal cell number and level of H(+)/K(+)-ATPase messenger RNA (mRNA) were reduced in the mutant strains, but gastrin-CCK double-KO mice displayed more active parietal cells and larger acid output than the gastrin KO mice. The acid response to histamine in double-KO mice was unchanged whereas that to gastrin was diminished, but it could be restored by infusion of gastrin. Oxyntic D-cell density was the same in both mutant strains, but the D cells were more active in the gastrin KO than in the double-KO mice. CCK infusion in gastrin-CCK double-KO mice raised the somatostatin mRNA level and inhibited acid secretion to the level seen in gastrin KO mice. Vagotomy and atropine abolished acid secretion in all 3 groups of mice. CONCLUSIONS: Lack of gastrin impairs the gastrin-ECL axis, whereas lack of gastrin and CCK impairs both hormonal pathways. In the gastrin-CCK double-KO mice, acid secretion is only controlled by cholinergic vagal stimulation, which normalizes the acid output. |
| | |
Authors:
|
Duan Chen; Chun-Mei Zhao; Rolf Håkanson; Linda C Samuelson; Jens F Rehfeld; Lennart Friis-Hansen |
Related Documents
:
|
18604505 - Two forms of vibrio vulnificus metalloprotease vvpe are secreted via the type ii genera... 3119235 - Circannual and circa-hemiannual rhythms of basal and stimulated gastric secretion in co... 602875 - The effect of hexamethonium on gastric acid secretion in the conscious rat. 17768875 - Defensive secretion of a caterpillar (papilio). 8763205 - Characterization of carbon paste electrodes in vitro for simultaneous amperometric meas... 17184455 - Prospective randomised single-blind controlled trial of glacial acetic acid versus glac... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Gastroenterology Volume: 126 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2004 Feb |
Date Detail:
|
Created Date: 2004-02-05 Completed Date: 2004-03-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
|
Languages: eng Pagination: 476-87 Citation Subset: AIM; IM |
Affiliation:
|
Department of Cancer Research & Molecular Medicine, Norweigian University of Science and Technology, Trondheim, Norway. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cholecystokinin / deficiency* Enterochromaffin Cells / secretion Gastric Acid / secretion* Gastrins / deficiency* Mice Mice, Knockout Parietal Cells, Gastric / secretion Somatostatin-Secreting Cells / secretion Vagotomy |
| Chemical | |
Reg. No./Substance:
|
0/Gastrins; 9011-97-6/Cholecystokinin |
| Comments/Corrections | |
Comment In:
|
Gastroenterology. 2004 Feb;126(2):606-9
[PMID:
14762799
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Improved clinical outcome using polytetrafluoroethylene-coated stents for TIPS: results of a randomi...
Next Document: High-molecular-weight polyethylene glycol prevents lethal sepsis due to intestinal Pseudomonas aerug...