Document Detail

Altered control of gastric acid secretion in gastrin-cholecystokinin double mutant mice.
MedLine Citation:
PMID:  14762785     Owner:  NLM     Status:  MEDLINE    
BACKGROUND & AIMS: Three pathways control gastric acid secretion: the gastrin-enterochromaffin-like (ECL) cell axis, the vagus-parietal cell axis, and the cholecystokinin (CCK)-D cell axis. Mice lacking gastrin or both gastrin and CCK were examined to determine the role of the hormones. METHODS: Acid was measured after pylorus ligation, and biopsies from gastrin knockout (KO), gastrin-CCK double-KO, and wild-type (WT) mice were collected for biochemical, immunocytochemical, and electron-microscopic examination. RESULTS: The ECL cells were inactive in both groups of mutant mice but the cell number was unaffected. Both parietal cell number and level of H(+)/K(+)-ATPase messenger RNA (mRNA) were reduced in the mutant strains, but gastrin-CCK double-KO mice displayed more active parietal cells and larger acid output than the gastrin KO mice. The acid response to histamine in double-KO mice was unchanged whereas that to gastrin was diminished, but it could be restored by infusion of gastrin. Oxyntic D-cell density was the same in both mutant strains, but the D cells were more active in the gastrin KO than in the double-KO mice. CCK infusion in gastrin-CCK double-KO mice raised the somatostatin mRNA level and inhibited acid secretion to the level seen in gastrin KO mice. Vagotomy and atropine abolished acid secretion in all 3 groups of mice. CONCLUSIONS: Lack of gastrin impairs the gastrin-ECL axis, whereas lack of gastrin and CCK impairs both hormonal pathways. In the gastrin-CCK double-KO mice, acid secretion is only controlled by cholinergic vagal stimulation, which normalizes the acid output.
Duan Chen; Chun-Mei Zhao; Rolf Håkanson; Linda C Samuelson; Jens F Rehfeld; Lennart Friis-Hansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gastroenterology     Volume:  126     ISSN:  0016-5085     ISO Abbreviation:  Gastroenterology     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-05     Completed Date:  2004-03-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374630     Medline TA:  Gastroenterology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  476-87     Citation Subset:  AIM; IM    
Department of Cancer Research & Molecular Medicine, Norweigian University of Science and Technology, Trondheim, Norway.
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MeSH Terms
Cholecystokinin / deficiency*
Enterochromaffin Cells / secretion
Gastric Acid / secretion*
Gastrins / deficiency*
Mice, Knockout
Parietal Cells, Gastric / secretion
Somatostatin-Secreting Cells / secretion
Reg. No./Substance:
0/Gastrins; 9011-97-6/Cholecystokinin
Comment In:
Gastroenterology. 2004 Feb;126(2):606-9   [PMID:  14762799 ]

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