Document Detail

Altered chemotactic behavior of crevicular PMNs in different forms of periodontitis.
MedLine Citation:
PMID:  11168741     Owner:  NLM     Status:  MEDLINE    
BACKGROUND, AIMS: Polymorphonuclear neutrophils (PMN) are the predominant host defence cells in the gingival sulcus. Previous work demonstrates that the in vitro phagocytosis of crevicular cells in localised early onset periodontitis (LEOP) and generalised early onset periodontitis (GEOP) lesions is diminished. The present study extends this work by characterizing the chemotaxis function of crevicular fluid (CF) PMNs in various forms of periodontitis. METHODS: We investigated 7 patients with LEOP, 11 patients with GEOP, 12 patients with adult periodontitis (AP) and 2 age- and sex-matched healthy control groups. The two deepest sites of each quadrant in test and control subjects were selected for crevicular sampling. Chemotaxis was performed in a micro chamber (moist atmosphere, 5% CO2, 37 degrees C, 30 min) using N-formyl-methionyl-leucyl-phenylalanine (FMLP, 1 x 10(-7) mol FMLP/l) as a chemoattractant. The total chemotaxis was defined as the difference between the number of cells migrating towards FMLP minus the number of cells migrating towards PBS, counted in 20 randomly selected fields. Membranes were examined microscopically at 400 x magnification. RESULTS: The chemotactic activity in the adult periodontitis group was significantly higher compared to the age-related control group. However, we found a statistically significant reduction of chemotactic activity in LEOP and GEOP patients compared to the controls. CONCLUSIONS: These results indicate an increase of chemotactic activity from CF-PMN in patients with adult periodontitis, but on the other hand, a significant reduction of chemotactic responsiveness of these cells in LEOP and GEOP lesions.
B Sigusch; S Eick; W Pfister; G Klinger; E Glockmann
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical periodontology     Volume:  28     ISSN:  0303-6979     ISO Abbreviation:  J. Clin. Periodontol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-01-30     Completed Date:  2001-03-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0425123     Medline TA:  J Clin Periodontol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  162-7     Citation Subset:  D; IM    
Department of Periodontology, Conservative Dentistry, Friedrich Schiller University of Jena, Germany.
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MeSH Terms
Actinobacillus actinomycetemcomitans / immunology
Aggressive Periodontitis / immunology
Case-Control Studies
Chemotaxis, Leukocyte / immunology*
Dental Plaque / microbiology
Dental Plaque Index
Gingival Crevicular Fluid / immunology*
Middle Aged
N-Formylmethionine Leucyl-Phenylalanine
Neutrophils / physiology
Periodontitis / immunology*
Statistics, Nonparametric
Reg. No./Substance:
59880-97-6/N-Formylmethionine Leucyl-Phenylalanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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