| Altered Turnover of {beta}-Carotene and Chlorophyll a in Arabidopsis Leaves Treated with Lincomycin or Norflurazon. | |
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MedLine Citation:
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PMID: 21632655 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Interactions between β-carotene (β-C) and chlorophyll a (Chl a) turnover were investigated in relation to photoinhibition and D1 protein turnover in mature leaves of Arabidopsis (Arabidopsis thaliana) by (14)CO(2) pulse-chase labeling. Following a 2-h treatment of leaves with water, lincomycin (Linco; inhibitor of chloroplast protein synthesis) or norflurazon (NF; inhibitor of carotenoid biosynthesis at phytoene desaturation) in dark, (14)CO(2) was applied to the leaves for 30 min under control light (CL; 130 µmol photons m-2 s-1) conditions, followed by exposure to either CL or high light (HL; 1100 µmol photons m(-2) s(-1)) in ambient CO(2) for up to 6 h. Under both light conditions, (14)C incorporation was strongly decreased for Chl a and moderately suppressed for β-C in Linco-treated leaves showing a marked decline of photosystem II efficiency (Fv/Fm) and β-C content compared to water-treated leaves. Partial inhibition of carotenoid biosynthesis by NF caused no or only a minor decrease of Fv/Fm and Chl a turnover under both conditions while β-C content significantly declined and high (14)C labeling was found for phytoene, the substrate of phytoene desaturase. Together, the results suggest coordinated turnover of Chl a and D1, but somewhat different regulation for β-C turnover, in Arabidopsis leaves. Inhibition of carotenoid biosynthesis by NF may initially enhance metabolic flux in the pathway upstream of phytoene, presumably compensating for short supply of β-C. Our observations are also in line with the notion that HL-induced accumulation of xanthophylls may involve a precursor pool which is distinct from the one for β-C turnover. |
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Authors:
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Kim Gabriele Beisel; Ulrich Schurr; Shizue Matsubara |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-1 |
Journal Detail:
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Title: Plant & cell physiology Volume: - ISSN: 1471-9053 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-2 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9430925 Medline TA: Plant Cell Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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IBG-2: Pflanzenwissenschaften, Forschungszentrum Jülich, 52425 Jülich, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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