Document Detail


Altered RNA editing of serotonin 2C receptor in a rat model of depression.
MedLine Citation:
PMID:  16005997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Altered RNA editing of serotonin 2C receptor (HTR2C) has been suggested to be involved in the pathophysiology of major depression. Here we examined RNA editing status of HTR2C in the learned helplessness (LH) rats, one of well-established animal models of depression. LH rats showed the significantly increased RNA editing of site E, and tendency for increased RNA editing of other editing sites. Treatment with fluoxetine, a selective serotonin reuptake inhibitor, or imipramine, a tricyclic antidepressant, affected the RNA editing status of the LH rats. Although, these antidepressants differentially altered RNA editing status, they commonly reduced RNA editing efficiency of site E. We further revealed that altered RNA editing in the LH rats and by antidepressants was not explained by altered expression of RNA editing enzymes or their substrates (adenosine deaminases that act on RNA, HTR2C, and spliced form of HTR2C). These results suggest that alteration of RNA editing of HTR2C may play a role in the pathophysiology of depression and action of antidepressants.
Authors:
Kazuya Iwamoto; Noriaki Nakatani; Miki Bundo; Takeo Yoshikawa; Tadafumi Kato
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience research     Volume:  53     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-01     Completed Date:  2005-10-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  69-76     Citation Subset:  IM    
Affiliation:
Laboratory for Molecular Dynamics of Mental Disorders, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako city, Saitama 351 0198, Japan. kaziwamoto@brain.riken.go.jp
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MeSH Terms
Descriptor/Qualifier:
Adenosine Deaminase / genetics,  metabolism
Animals
Antidepressive Agents / therapeutic use
Cloning, Molecular
Depression / drug therapy,  genetics*
Disease Models, Animal
Gene Expression Regulation / drug effects,  physiology
Genetic Variation / physiology
Helplessness, Learned*
Male
RNA Editing*
RNA, Messenger / metabolism
Rats
Rats, Sprague-Dawley
Receptor, Serotonin, 5-HT2C / genetics*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction / methods
Sequence Analysis / methods
Chemical
Reg. No./Substance:
0/Antidepressive Agents; 0/RNA, Messenger; 0/Receptor, Serotonin, 5-HT2C; EC 3.5.4.-/dsRNA adenosine deaminase; EC 3.5.4.4/Adenosine Deaminase

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