Document Detail

Altered Na(+)- H(+)- exchange activity in the spontaneously hypertensive perfused rat heart.
MedLine Citation:
PMID:  8523411     Owner:  NLM     Status:  MEDLINE    
Intracellular pH (pHi) and developed pressure during hypercapnic acidosis were studied in the spontaneously hypertensive rat (SHR) heart and Wistar-Kyoto controls. Developed pressure was determined using a modified Langendorff isovolumic perfusion technique and 31P magnetic resonance spectroscopy was used to determine pHi. In response to acidosis, both developed pressure and pHi first decreased and then partially recovered. In the SHR group, pHi during the early periods of acidosis was significantly higher than in the control group while there was no significant difference in the steady-state pHi. The addition of 5-(N,N)-hexamethylene-amiloride (HMA), a specific inhibitor of Na(+)-H+ exchange, abolished these difference. Furthermore, HMA was found to inhibit recovery in pHi and developed pressure during acidosis in both groups. These results demonstrate that Na(+)-H(+)-exchange in the rat heart plays a major role in pHi regulation and contributes to functional recovery during acidosis. In addition, Na(+)-H(+)-exchange activity, as previously found in other tissues in hypertension, is increased in the SHR heart.
A E Schussheim; G K Radda
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  27     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1996-01-22     Completed Date:  1996-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1475-81     Citation Subset:  IM    
Department of Biochemistry, University of Oxford, UK.
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MeSH Terms
Amiloride / analogs & derivatives,  pharmacology
Carbon Dioxide / blood
Heart / drug effects
Hydrogen-Ion Concentration*
Hypertension / genetics,  metabolism*
Myocardium / metabolism*
Rats, Inbred SHR
Rats, Inbred WKY
Sodium / metabolism*
Sodium-Hydrogen Antiporter / metabolism*
Time Factors
Reg. No./Substance:
0/Sodium-Hydrogen Antiporter; 124-38-9/Carbon Dioxide; 1428-95-1/5-(N,N-hexamethylene)amiloride; 2609-46-3/Amiloride; 7440-23-5/Sodium

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