Document Detail


Altered MicroRNA Expression Profiles in Retinas with Diabetic Retinopathy.
MedLine Citation:
PMID:  22156553     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Rats with streptozotocin (STZ)-induced diabetes were studied in order to identify abnormal microRNA (miRNA) expression profiles in diabetic retinopathy (DR) and to ascertain miRNAs associated with DR. Histopathologically, we observed characteristic features of DR in rats at 10 weeks after STZ injection. Investigation of miRNA expression profiles in the retinas of control and diabetic rats using miRNA microarrays revealed that many miRNAs were abnormally expressed in DR. On the basis of their fold changes and probability values, a total of 37 miRNAs were selected for further validation by real-time PCR analysis. The results showed that 11 miRNAs were significantly upregulated and 6 miRNAs were notably downregulated in DR. Furthermore, these changes in retinal miRNA expression levels paralleled the course of DR. Levels of miR-182, miR-96, miR-183, miR-211, miR-204, and miR-124 were significantly increased during the progress of DR, whereas miR-10b, miR-10a, miR-219-2-3p, miR-144, miR-338, and miR-199a-3p were significantly decreased. Our data indicate that the aberrant miRNA expression profiles in DR are associated with the development of DR. Modulation of retinal miRNA expression levels may provide a potential therapeutic strategy for DRs.
Authors:
Jin-Hui Wu; Yu Gao; An-Jing Ren; Shi-Hong Zhao; Ming Zhong; Ya-Jun Peng; Wei Shen; Ming Jing; Lin Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-08
Journal Detail:
Title:  Ophthalmic research     Volume:  47     ISSN:  1423-0259     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0267442     Medline TA:  Ophthalmic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  195-201     Citation Subset:  -    
Copyright Information:
Copyright © 2011 S. Karger AG, Basel.
Affiliation:
Department of Ophthalmology at Changhai Hospital, Shanghai, China.
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