Document Detail


Altered fate of subventricular zone progenitor cells and reduced neurogenesis following neonatal stroke.
MedLine Citation:
PMID:  20453463     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effects of neonatal stroke on progenitor cells lining the lateral ventricles. METHODS: Intraventricular injection of replication-incompetent green fluorescent protein (GFP)-expressing lentivirus was performed in postnatal day 1 (P1) rats to specifically label radial glia/type B neural stem cells and ependymal cells of the lateral ventricle. A subset of animals was exposed to transient middle cerebral artery occlusion (MCAO) at P7, with mild or moderate injury confirmed by diffusion-weighted MRI and histology. Newborn cells were identified by GFP expression, location and expression of cell type-specific markers in the striatum, cortex and olfactory bulb using confocal microscopy and systematic random sampling. RESULTS: Three weeks lentiviral GFP transduction of cells in the lateral ventricle, abundant GFP-expressing neurons and glia were identified in the rostral migratory stream, olfactory bulb and striatum as expected from labeling the subventricular zone (SVZ) type B neural stem cell lineage. Two weeks following mild or severe focal stroke at P7, no GFP-expressing neurons were detected in striatum or cortex although some single-labeled doublecortin+ immature neurons were detected in the penumbra. The densities of GFP+/ glial fibrillary acidic protein (GFAP)+ astrocytes and GFP+/O4+ oligodendrocytes were reduced in the striatum following MCAO (4.8 +/- 1.02 vs. 2.5 +/- 0.4 cells/high-power field, HPF; p = 0.005; 2.8+/- 1 vs. 0.5 +/- 0.2 cells/HPF, p = 0.008). Furthermore, there was a reduction of GFP+ cells in the olfactory bulb following MCAO (58.8 +/- 14.9 vs. 19.6 +/- 5.4 cells/HPF, p = 0.025). Finally, there was an increased percentage of GFP+/GFAP+ cells (70 vs. 50%), with a decreased proportion of GFP+/O4+ cells (14 vs. 30%) in injured animals. CONCLUSION: Neurogenesis originating from cells of the lateral ventricle, including SVZ type B cells, is significantly reduced following neonatal stroke. Furthermore, neonatal stroke disrupts gliogenesis in the striatum, decreasing overall numbers of new glia and shifting the population towards astrocytes.
Authors:
Ruggero Spadafora; Fernando F Gonzalez; Nikita Derugin; Michael Wendland; Donna Ferriero; Patrick McQuillen
Related Documents :
18789733 - Central nervous system extracellular matrix changes in a transgenic mouse model of bovi...
19635473 - Shp2 acts downstream of sdf-1alpha/cxcr4 in guiding granule cell migration during cereb...
19550143 - 1-o-hexadecyl-2-o-methyl-3-o-(2'-acetamido-2'-deoxy-beta-d-glucopyranosyl)-sn-glycerol ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-04
Journal Detail:
Title:  Developmental neuroscience     Volume:  32     ISSN:  1421-9859     ISO Abbreviation:  Dev. Neurosci.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-10-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7809375     Medline TA:  Dev Neurosci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  101-13     Citation Subset:  IM    
Copyright Information:
Copyright 2010 S. Karger AG, Basel.
Affiliation:
Department of Neurology, University of California, San Francisco, CA 94143-0663, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn*
Biological Markers / metabolism
Ependyma / cytology,  metabolism
Genetic Vectors / genetics,  metabolism
Green Fluorescent Proteins / genetics,  metabolism
Humans
Infarction, Middle Cerebral Artery
Lateral Ventricles* / cytology,  metabolism
Lentivirus / genetics,  metabolism
Neurogenesis / physiology*
Neuroglia / cytology,  metabolism
Olfactory Receptor Neurons / cytology,  metabolism
Rats
Rats, Long-Evans
Stem Cells / physiology*
Stroke* / pathology,  physiopathology
Chemical
Reg. No./Substance:
0/Biological Markers; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Negative Correlation between Neuropeptide Y Profile in the Cerebrospinal Fluid and Growth Hormone Pu...
Next Document:  Expression of Synaptic Proteins in the Hippocampus and Spatial Learning in Chicks following Prenatal...