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Altered Expression of Fas Receptor on Alveolar Macrophages and Inflammatory Effects of Soluble Fas Ligand Following Blunt Chest Trauma.
MedLine Citation:
PMID:  21330946     Owner:  NLM     Status:  Publisher    
Blunt chest trauma impairs the outcome of multiple injured patients. Lung contusion induces inflammatory alterations and Fas-dependent apoptosis of alveolar epithelial type 2 (AT2) cells has been described. The Fas/Fas ligand (FasL) system seems to exhibit a proinflammatory potential. We aimed to elucidate the involvement of the Fas/FasL system in the inflammatory response after lung contusion.Chest trauma was induced in male rats by a pressure wave. Soluble FasL Concentrations were determined in bronchoalveolar lavage fluids (BAL) and alveolar macrophage (AMΦ) supernatants. AMΦ and AT2 cells were isolated to determine the surface expression (FACS) of Fas/FasL, the mRNA expression (RT-PCR) of Fas, FasL, TNF-α, IL-6 and IL-10 and to measure the release of IL-6 and IL-10 after culture with or without stimulation with FasL.After chest trauma, FasL concentration was increased in BAL and AMΦ supernatants and Fas and FasL protein was down-regulated on AMΦ and unchanged on AT2 cells. The mRNA expression of Fas was increased in AMΦ and AT2 cells and that of FasL only in AMΦ isolated after lung contusion. FasL stimulation further enhanced IL-6 and suppressed IL-10 release in AMΦ after trauma.The results indicate that the Fas/FasL system is activated after chest trauma and FasL is associated with the inflammatory response after lung contusion. The pro-inflammatory response of AMΦ is enhanced by FasL stimulation. Both AMΦ and AT2 cells seem to contribute to the mediator release after lung contusion. These results confirm the importance of the Fas/FasL system in the inflammatory response after chest trauma.
Daniel H Seitz; Annette Palmer; Ulrike Niesler; Sonja T Braumüller; Simon Bauknecht; Florian Gebhard; Markus W Knöferl
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-15
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  -     ISSN:  1540-0514     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Trauma Surgery, Hand, Plastic and Reconstructive Surgery, University of Ulm, Steinhövelstr. 9, 89075 Ulm, Germany.
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