| Alterations of ovariectomized rat bone and impact of non-collagenous proteins on mineralization. | |
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MedLine Citation:
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PMID: 19059798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: Noncollagenous proteins (NCPs) play distinct and overlap roles in controlling the mineralization of bone, while the relationship between the NCPs and the mineral phase in osteoporotic bone is nebulous. Ovariectomized (OVX) rats, as an animal model for osteopenia, were used in this study to evaluate the impact of NCPs on mineral metabolism in osteopenia development. METHODS: Bone mineral was characterized with small-angle X-ray scattering study, extended X-ray absorption fine structure analysis, inductively coupled plasma mass spectroscopy, thermogravimetric and chemical analyses. NCPs were extracted from bone matrix, and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). A constant composition titration system was used in the kinetic study of re-mineralization of bone residues after various extractions. RESULTS: Bone density was reduced, whereas no significant changes were observed in the composition, crystal thickness and crystal structural parameters of minerals from OVX rat bone. The total content of NCPs in each extract decreased in the OVX group, but no selective loss of particular proteins was observed. The difference in mineralization kinetics between the OVX rat bone and the sham rat bone was resulted mainly from the mineral-bound NCPs. CONCLUSIONS: The reduced quantity and unchanged quality of bone mineral seem to be positively associated with the total amount of NCPs and the proportions between some major NCPs in OVX rats, respectively. |
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Authors:
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Jian Huang; Xi Wang; Tian-Lan Zhang; Kui Wang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-06 |
Journal Detail:
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Title: Joint, bone, spine : revue du rhumatisme Volume: 76 ISSN: 1778-7254 ISO Abbreviation: Joint Bone Spine Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-09 Completed Date: 2009-06-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100938016 Medline TA: Joint Bone Spine Country: France |
Other Details:
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Languages: eng Pagination: 176-83 Citation Subset: IM |
Affiliation:
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Department of Chemical Biology, Peking University School of Pharmaceutical Sciences, 38 Xueyuan Road, Beijing 100191, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Animals Bone Density Bone Diseases, Metabolic / etiology, metabolism, pathology* Bone and Bones / chemistry, metabolism Calcification, Physiologic / physiology* Disease Models, Animal Electrophoresis, Polyacrylamide Gel Extracellular Matrix Proteins / chemistry, metabolism* Female Mass Spectrometry Ovariectomy Rats Rats, Sprague-Dawley Scattering, Small Angle |
| Chemical | |
Reg. No./Substance:
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0/Extracellular Matrix Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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