Document Detail


Alterations in peroxisome proliferator-activated receptor mRNA expression in skeletal muscle after acute and repeated bouts of exercise.
MedLine Citation:
PMID:  19588229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxisome proliferator-activated receptors (PPAR) exist in three different forms, alpha (alpha), beta/delta (beta/delta), or gamma (gamma), all of which are expressed in skeletal muscle and play a critical role in the regulation of oxidative metabolism. The purpose of this investigation was to determine the mRNA expression pattern of the different PPARs and peroxisome proliferator-activated receptor alpha coactivator-1 alpha (PGC-1alpha) in muscles that largely rely on either glycolytic (plantaris) or oxidative (soleus) metabolism. Further, we also examined the alterations in the PPARs mRNA expression after one bout of endurance exercise or after 12 weeks of exercise training in the different muscles. Female Sprague-Dawley rats (5-8 months) were either run on the treadmill once or exercised trained for 12 weeks. The muscles were removed 24 h after the last bout of exercise. The results demonstrated with the exception of PPAR beta/delta, the PPAR mRNAs are expressed to a greater extent in the soleus muscle than in the plantaris muscle in sedentary animals. PPARgamma was the least abundantly expressed PPAR in either the soleus or the plantaris muscle. With respect to exercise training, only PPARgamma mRNA expression increased in the soleus muscle, while PPARbeta/delta and gamma mRNA levels increased in the plantaris muscle. Minimal changes were detected in any of the PPARs with one bout of exercise training. These results suggest that PPARgamma mRNA levels are the lowest in skeletal muscle among all of the PPARs and PPARgamma mRNA is the most responsive to changes in physical activity levels.
Authors:
Espen E Spangenburg; David A Brown; Micah S Johnson; Russell L Moore
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-07-09
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  332     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-05     Completed Date:  2010-03-04     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  225-31     Citation Subset:  IM    
Affiliation:
Department of Kinesiology, University of Maryland, College Park, MD 20742, USA. espen@umd.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Citrate (si)-Synthase / genetics,  metabolism
Female
Muscle, Skeletal / physiology*
Peroxisome Proliferator-Activated Receptors / genetics*,  metabolism
Physical Conditioning, Animal / physiology*
Physical Endurance
RNA, Messenger / genetics*,  metabolism
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
HL 40306-15/HL/NHLBI NIH HHS; HL 72790-02/HL/NHLBI NIH HHS; R01 HL040306-15/HL/NHLBI NIH HHS; R01 HL072790-02/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Peroxisome Proliferator-Activated Receptors; 0/RNA, Messenger; EC 2.3.3.1/Citrate (si)-Synthase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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