Document Detail

Alterations in mouse embryo intracellular pH by DMO during culture impair implantation and fetal growth.
MedLine Citation:
PMID:  20615756     Owner:  NLM     Status:  MEDLINE    
The preimplantation embryo is highly susceptible to in-vitro stress, and although this does not necessarily perturb blastocyst development, it can significantly affect embryo physiology and the ability to form a viable pregnancy. This study determined that the preimplantation mouse embryo is highly sensitive to a small decrease in intracellular pH (<0.2 pH units). Embryos cultured in media containing a weak acid (5,5-dimethyl-2,4-oxazolidinedione; DMO) formed blastocysts with decreased cell number and inner cell mass number, as well as increased apoptosis, even though blastocyst development and morphology were unchanged. Interestingly, the effects were similar regardless of whether the pH stress was present for a short-term 'acute' exposure (during the zygote to 2-cell, or 2-cell to 8-cell division) or an extended 'chronic' period of time (continually from the zygote to the blastocyst stage). Exposure to DMO during the first cleavage division did not alter implantation; however, fetal weight and crown-rump length were significantly decreased (P<0.05). In contrast, continuous exposure to DMO throughout preimplantation development reduced not only implantation but also fetal weight and crown-rump length. This study highlights the importance of correct intracellular pH and demonstrates that slight deviations can significantly impact embryo development and viability.
Deirdre L Zander-Fox; Megan Mitchell; Jeremy G Thompson; Michelle Lane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-05
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  21     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-04     Completed Date:  2010-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  219-29     Citation Subset:  IM    
Copyright Information:
2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
Research Centre for Reproductive Health, Discipline of Obstetrics and Gynaecology, University of Adelaide, South Australia, Australia.
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MeSH Terms
Dimethadione / pharmacology*
Embryo Implantation / drug effects*
Embryonic Development / drug effects*
Hydrogen-Ion Concentration*
Mice, Inbred C57BL
Mice, Inbred CBA
Reg. No./Substance:

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