Document Detail

Alterations in the morphology of Bacillus subtilis after exposure to beta-lysin and ultraviolet light.
MedLine Citation:
PMID:  4967778     Owner:  NLM     Status:  MEDLINE    
Viable counts, turbidities, and electron micrographs of Bacillus subtilis exposed to beta-lysin and ultraviolet light (UV), singly or in combination, were compared in an attempt to relate death with changes in morphology. The decreases in survival of both the beta-lysin- and UV-treated cells were rapid and preceded decreases in turbidity, as well as the changes in morphology. No significant differences were observed in turbidity reduction or morphological alterations of control cells from those of cells exposed to UV light. These cells developed prominent subcell wall spaces during incubation in the hypertonic stabilizing medium. No observable damage in either the cell wall or the cell membrane had taken place during 4 hr, but by 20 hr extensive damage of these two structures was apparent. The control and UV-treated cells exposed to beta-lysin did not develop prominent subcell wall spaces. Within 2 hr, lesions were observable in their cell walls, and the cytoplasmic membranes were permeable to phosphotungstic acid. The damage to these structures became more extensive with time. Although the visible changes of control and UV-treated cells were evident much later than those induced by beta-lysin, the morphological alterations in all cells were similar. It appeared that beta-lysin caused an accelerated release of an autolytic enzyme which digested the cell walls.
A Matheson; D M Donaldson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  95     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 May 
Date Detail:
Created Date:  1968-07-09     Completed Date:  1968-07-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1892-902     Citation Subset:  IM    
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MeSH Terms
Bacillus subtilis / cytology,  drug effects*,  radiation effects*
Blood Bactericidal Activity
Cell Membrane / drug effects
Cell Wall / drug effects
Microscopy, Electron
Radiation Effects*
Ultraviolet Rays*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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