Document Detail

Alterations in mRNA expression of ribosomal protein S9 in hydrogen peroxide-treated neurotumor cells and in rat hippocampus after transient ischemia.
MedLine Citation:
PMID:  12718447     Owner:  NLM     Status:  MEDLINE    
This study was designed to isolate new genes related to apoptosis in rat pheochromocytoma (PC12) cells treated with hydrogen peroxide (H2O2), and to characterize the roles of the genes using both in vitro and in vivo models of oxidative injury. cDNA libraries were prepared from H2O2-treated and -untreated PC12 cells, and a ribosomal protein S9 (RPS9) clone was isolated by a differential screening method. Increase of RPS9 expression in both H2O2-treated PC12 and neuroblastoma (Neuro-2A) cells was shown by Northern blot analysis. Viability of the antisense-transfected Neuro-2A (RPS9-AS) cells following H2O2 treatment was significantly reduced in a dose-dependent manner. In an in vivo model of transient forebrain ischemia, an increase in RPS9 expression was prominent by 1 day postischemia in the granule cell layer neurons of the dentate gyrus. Both activation of caspase-3 and significant recovery of viability following pretreatment with cycloheximide were shown in RPS9-AS cells treated with H2O2. These data suggest that RPS9 plays a protective role in oxidative injury of neuronal cells.
Seong Yun Kim; Mun-Yong Lee; Kyu Cheol Cho; Yun-Sik Choi; Jeong-Sun Choi; Ki-Wug Sung; Oh-Joo Kwon; Ho-Sik Kim; In-Kyung Kim; Seong-Whan Jeong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-04-29     Completed Date:  2003-07-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  925-31     Citation Subset:  IM    
Department of Pharmacology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
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MeSH Terms
Adrenal Gland Neoplasms
Cell Line
Cell Survival / drug effects
Gene Expression Regulation / drug effects
Hippocampus / drug effects,  metabolism*
Hydrogen Peroxide / pharmacology*
In Situ Hybridization
Ischemic Attack, Transient / metabolism*
PC12 Cells
RNA, Messenger / drug effects,  genetics*
Ribosomal Proteins / genetics*
Reg. No./Substance:
0/RNA, Messenger; 0/Ribosomal Proteins; 0/ribosomal protein S9; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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