Document Detail

Alterations in the levels of expression of specific cellular genes in adenovirus-infected and -transformed cells.
MedLine Citation:
PMID:  1441738     Owner:  NLM     Status:  MEDLINE    
There is increasing evidence that changes in the transcriptional program of cellular genes in virus-transformed cells can contribute to virus transformation. It is, therefore, important to study altered expression patterns of cellular genes in adenovirus-infected and -transformed cells. We have used 40 different cellular genes or gene segments as hybridization probes to analyze the cytoplasmic RNA from adenovirus type 2 (Ad2)-infected KB cells, from Ad5-transformed human cells (293) or from several Ad2- or adenovirus type 12 (Ad12)-transformed hamster cell lines. Many of the genes probed were not expressed in human or hamster cells. Transcription of the ADPRT and the heat shock protein 70 genes was increased in Ad2-infected KB cells and in 293 cells. In Ad2-infected KB cells, c-myc gene transcription was decreased. In 293 cells and in three adenovirus-transformed hamster cell lines (T637, BHK21-Ad2E1A-E1B, and BHK21-Ad2 HindIII-G), the transcription of the c-jun gene was increased, whereas c-myc transcription was decreased in the latter two cell lines. The data presented here demonstrate that, among 40 different mammalian gene probes, alterations in steady state levels of RNA were detected for five of these genes. These results suggest major alterations in transcription patterns in adenovirus-infected and -transformed cells.
T Rosahl; W Doerfler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  26     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  71-90     Citation Subset:  IM    
Institute for Genetics, University of Cologne, Germany.
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MeSH Terms
Actins / genetics
Adenoviridae / genetics*
Cell Line
Cell Line, Transformed
Cell Transformation, Viral*
Gene Expression Regulation*
Genes, jun
Genes, myc
Genome, Viral*
Heat-Shock Proteins / genetics
KB Cells
Poly(ADP-ribose) Polymerases / genetics*
RNA, Messenger / genetics,  metabolism
Transcription, Genetic
Tubulin / genetics
Reg. No./Substance:
0/Actins; 0/Heat-Shock Proteins; 0/RNA, Messenger; 0/Tubulin; EC Polymerases

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