Document Detail


Alterations in the levels of expression of specific cellular genes in adenovirus-infected and -transformed cells.
MedLine Citation:
PMID:  1441738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is increasing evidence that changes in the transcriptional program of cellular genes in virus-transformed cells can contribute to virus transformation. It is, therefore, important to study altered expression patterns of cellular genes in adenovirus-infected and -transformed cells. We have used 40 different cellular genes or gene segments as hybridization probes to analyze the cytoplasmic RNA from adenovirus type 2 (Ad2)-infected KB cells, from Ad5-transformed human cells (293) or from several Ad2- or adenovirus type 12 (Ad12)-transformed hamster cell lines. Many of the genes probed were not expressed in human or hamster cells. Transcription of the ADPRT and the heat shock protein 70 genes was increased in Ad2-infected KB cells and in 293 cells. In Ad2-infected KB cells, c-myc gene transcription was decreased. In 293 cells and in three adenovirus-transformed hamster cell lines (T637, BHK21-Ad2E1A-E1B, and BHK21-Ad2 HindIII-G), the transcription of the c-jun gene was increased, whereas c-myc transcription was decreased in the latter two cell lines. The data presented here demonstrate that, among 40 different mammalian gene probes, alterations in steady state levels of RNA were detected for five of these genes. These results suggest major alterations in transcription patterns in adenovirus-infected and -transformed cells.
Authors:
T Rosahl; W Doerfler
Related Documents :
9367888 - Efficient transfer of genes into senescent cells by adenovirus vectors via highly expre...
11292568 - Tetracycline-regulatable adenovirus vectors: pharmacologic properties and clinical pote...
16497398 - X-gal inhibits the swarming of vibrio species.
7729028 - In vivo gene transfer into injured carotid arteries. optimization and evaluation of acu...
15925458 - Vectors that facilitate the replacement of transcriptional lacz fusions in streptococcu...
25410248 - An integrated "omics" approach to the characterization of maize (zea mays l.) mutants d...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virus research     Volume:  26     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  71-90     Citation Subset:  IM    
Affiliation:
Institute for Genetics, University of Cologne, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actins / genetics
Adenoviridae / genetics*
Animals
Cell Line
Cell Line, Transformed
Cell Transformation, Viral*
Gene Expression Regulation*
Genes, jun
Genes, myc
Genome, Viral*
Heat-Shock Proteins / genetics
Humans
KB Cells
Poly(ADP-ribose) Polymerases / genetics*
RNA, Messenger / genetics,  metabolism
Transcription, Genetic
Tubulin / genetics
Chemical
Reg. No./Substance:
0/Actins; 0/Heat-Shock Proteins; 0/RNA, Messenger; 0/Tubulin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A carboxy-terminal mutant spleen focus-forming virus (SFFV) envelope glycoprotein is transport-compe...
Next Document:  Mercaptoethanol and dithiothreitol decrease the difference of electrochemical proton potentials acro...