| Alterations in hippocampal GAP-43, BDNF, and L1 following sustained cerebral ischemia. | |
| | |
MedLine Citation:
|
PMID: 12062469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Alterations in factors involved in the regeneration of the neuronal network in the hippocampus of rats with microsphere embolism (ME) were examined. Nine hundred microspheres (48 microm in diameter) were injected into the right hemisphere, and immunochemical and immunohistochemical studies on the hippocampus were performed on the seventh day thereafter. Hematoxylin-eosin staining showed progressive and severe degeneration of the hippocampus after ME. The protein levels of brain-derived neurotrophic factor (BDNF), 43-kDa growth-associated protein (GAP-43), and adhesion protein L1 (L1) in the ipsilateral hippocampus of the ME animal, determined by Western blot analysis or enzyme immunoassay, were increased, unaltered, and decreased, respectively. In contrast, the immunohistochemical study showed increases in a marker of axonal sprouting GAP-43, and a neurotrophic factor BDNF, and a decrease in an adhesion molecule L1 in some areas of the hippocampal ischemic penumbra of such animals. These results suggest that some factors for regeneration of the neuronal network in the ischemic penumbra responded to sustained cerebral ischemia for a certain period, although functional network of the nerve cells in the microsphere-injected hemisphere would be unlikely established after ME. |
| | |
Authors:
|
Keiko Miyake; Wataru Yamamoto; Mina Tadokoro; Norio Takagi; Kyoko Sasakawa; Atsumi Nitta; Shoei Furukawa; Satoshi Takeo |
Related Documents
:
|
7978479 - Minimum alveolar concentration for halothane in the rat is resistant to effects of fore... 7792009 - Cyclic gmp levels in the rat brain and plasma during clinical death and after resuscita... 16182259 - Bradykinin preconditioning induces protective effects against focal cerebral ischemia i... 9637399 - Postischemic treatment with calpain inhibitor mdl 28170 ameliorates brain damage in a g... 8434069 - Ameliorative effects of the centrally active cholinesterase inhibitor, nik-247, on impa... 19812919 - Reduced matrix metalloproteinase-9 activity and cell death after global ischemia in the... 1672899 - The influence of the gamma-amino butyric acid (gaba) antagonist bicuculline on transpor... 1871679 - Gaba depolarizes neurons in the rat striatum: an in vivo study. 22402319 - Effect of herpes simplex virus vector-mediated interleukin-4 gene therapy on bladder ov... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Brain research Volume: 935 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2002 May |
Date Detail:
|
Created Date: 2002-06-13 Completed Date: 2002-08-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 24-31 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, 192-0392 Hachioji, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain Ischemia / metabolism*, pathology, physiopathology Brain-Derived Neurotrophic Factor / metabolism* Carotid Artery Thrombosis / metabolism, pathology, physiopathology Cell Death / physiology Cerebral Infarction / metabolism*, pathology, physiopathology Cerebrovascular Circulation / physiology Disease Models, Animal Down-Regulation / physiology GAP-43 Protein / metabolism* Hippocampus / metabolism*, pathology, physiopathology Immunohistochemistry Leukocyte L1 Antigen Complex Male Membrane Glycoproteins / metabolism* Microspheres Nerve Degeneration / metabolism, pathology, physiopathology Nerve Regeneration / physiology* Neural Cell Adhesion Molecules / metabolism* Rats Rats, Wistar Time Factors Up-Regulation / physiology |
| Chemical | |
Reg. No./Substance:
|
0/Brain-Derived Neurotrophic Factor; 0/GAP-43 Protein; 0/Leukocyte L1 Antigen Complex; 0/Membrane Glycoproteins; 0/Neural Cell Adhesion Molecules |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Subcellular immunolocalization of NMDA receptor subunit NR1, 2A, 2B in the rat vestibular periphery.
Next Document: MPP+ increases alpha-synuclein expression and ERK/MAP-kinase phosphorylation in human neuroblastoma ...