Document Detail


Alterations in fatty acid kinetics in obese adolescents with increased intrahepatic triglyceride content.
MedLine Citation:
PMID:  18948971     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: It has been hypothesized that excessive fatty acid availability contributes to steatosis and the metabolic abnormalities associated with nonalcoholic fatty liver disease (NAFLD). The purpose of this study was to evaluate whether adipose tissue lipolytic activity and the rate of fatty acid release into plasma are increased in obese adolescents with NAFLD.
METHODS: Palmitate kinetics were determined in obese adolescents with normal (n = 9; BMI = 37 +/- 2 kg/m(2); intrahepatic triglyceride (IHTG) <or=5.5% of liver volume) and increased (n = 9; BMI = 36 +/- 2 kg/m(2); IHTG >or= 10% of liver volume) IHTG content during the basal state (postabsorptive condition) and during physiological hyperinsulinemia (postprandial condition). Both groups were matched on body weight, BMI, percent body fat, age, sex, and Tanner stage. The hyperinsulinemic-euglycemic clamp procedure, in conjunction with a deuterated palmitate tracer infusion, was used to determine free-fatty acid (FFA) kinetics, and magnetic resonance spectroscopy was used to determine IHTG content.
RESULTS: The rate of whole-body palmitate release into plasma was greater in subjects with NAFLD than those with normal IHTG content during basal conditions, (87 +/- 7 vs. 127 +/- 13 micromol/min; P < 0.01) and during physiological hyperinsulinemia, (24 +/- 2 vs. 44 +/- 8 micromol/min; P < 0.01).
DISCUSSION: These results demonstrate that adipose tissue lipolytic activity is increased in obese adolescents with NAFLD and results in an increase in the rate of fatty acid release into plasma throughout the day. This continual excess in fatty acid flux supports the hypothesis that adipose insulin resistance is involved in the pathogenesis of steatosis and contributes to the metabolic complications associated with NAFLD.
Authors:
Elisa Fabbrini; Dinky deHaseth; Sheela Deivanayagam; B Selma Mohammed; Bernadette E Vitola; Samuel Klein
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-10-23
Journal Detail:
Title:  Obesity (Silver Spring, Md.)     Volume:  17     ISSN:  1930-7381     ISO Abbreviation:  Obesity (Silver Spring)     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-24     Completed Date:  2009-03-24     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  101264860     Medline TA:  Obesity (Silver Spring)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Abdomen
Adipose Tissue / anatomy & histology
Adolescent
Body Composition
Body Mass Index
Fatty Liver / metabolism
Female
Glucose Clamp Technique
Humans
Hyperinsulinism
Liver / metabolism*
Magnetic Resonance Imaging
Male
Obesity / metabolism*
Palmitic Acid / blood,  metabolism*
Skin
Triglycerides / metabolism*
Grant Support
ID/Acronym/Agency:
DK 37948/DK/NIDDK NIH HHS; DK 56341/DK/NIDDK NIH HHS; KL2 RR024994/RR/NCRR NIH HHS; KL2 RR024994-02/RR/NCRR NIH HHS; P30 DK056341/DK/NIDDK NIH HHS; P30 DK056341-07/DK/NIDDK NIH HHS; P30 DK056341-08/DK/NIDDK NIH HHS; P41 RR000954/RR/NCRR NIH HHS; P41 RR000954-32/RR/NCRR NIH HHS; R01 DK037948/DK/NIDDK NIH HHS; R01 DK037948-22/DK/NIDDK NIH HHS; RR-00954/RR/NCRR NIH HHS; RR024992/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Triglycerides; 2V16EO95H1/Palmitic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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