Document Detail


Alterations in fatty acid composition of tissue phospholipids in the developing retinal dystrophic rat.
MedLine Citation:
PMID:  11768159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alterations in lipid composition occur in the retinal pigment epithelium and photoreceptor cells of the Royal College of Surgeons (RCS) dystrophic rat, a model for inherited retinal degeneration. With respect to lipid composition of nonretinal tissues, the developmental timing of lipid alterations and the incidence of dystrophy are unknown. We determined the fatty acid composition in choline phosphoglycerides (ChoGpl) and ethanolamine phosphoglycerides (EtnGpl) in the brain, liver, and retina from dystrophic RCS rats and from their nondystrophic congenics (controls) at the ages of 3 and 6 wk. At 3 wk, the fatty acid compositions were specific to individual phospholipid classes without any difference between dystrophic and nondystrophic tissues. In plasma phospholipids, there was an age-related increase in the relative contents of monounsaturated and n-3 polyunsaturated fatty acids, with only minor differences between dystrophic and nondystrophic rats. At 6 wk, the fatty acid compositions in ChoGpl and EtnGpl from dystrophic brain and retina were significantly different from those of nondystrophics. The effect of strain on developmental changes in brain fatty acid composition was significant for 18:0 and 22:6n-3 in EtnGpl and for 16:0, 18:0, 18:1n-9, and 20:4n-6 in ChoGpl. The brain ChoGpl fatty acid composition in nondystrophic rats was similar at 6 wk to that of normal rats, and there were almost no postweaning changes in the dystrophics. In retinal phospholipids, the effect of dystrophy was to increase the 20:4n-6 content in EtnGpl and to decrease 22:6n-3 in ChoGpl. The 18:2n-6 and 22:6n-3 contents in dystrophic liver ChoGpl were also significantly affected, while no difference was observed in the EtnGpl fraction. The dystrophy affected the phospholipid fatty acid developmental changes in a tissue- and class-specific manner. Fatty acid metabolism could be selectively altered in neural and nonneural tissues of developing dystrophic RCS rats.
Authors:
J M Alessandri; B Goustard-Langelier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  36     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-20     Completed Date:  2002-05-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1141-52     Citation Subset:  IM    
Affiliation:
Nutrition and Food Safety Laboratory, INRA, Jouy-en-Josas, France. alessand@diamant.jouy.inra.fr
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Brain Chemistry
Disease Models, Animal
Fatty Acids / analysis*
Fatty Acids, Monounsaturated / blood
Fatty Acids, Omega-3 / blood
Liver / chemistry
Phosphatidylcholines / analysis
Phosphatidylethanolamines / analysis
Phospholipids / blood,  chemistry*
Rats
Rats, Mutant Strains
Retina / chemistry
Retinal Degeneration / metabolism*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Omega-3; 0/Phosphatidylcholines; 0/Phosphatidylethanolamines; 0/Phospholipids
Comments/Corrections
Erratum In:
Lipids. 2003 Sep;38(9):1005

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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