Document Detail


Alterations in the expression and gating of Drosophila sodium channels by mutations in the para gene.
MedLine Citation:
PMID:  2560637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mutations in the para gene specifically affect the expression of sodium currents in Drosophila. While 65% of wild-type embryonic neurons in culture express sodium currents, three distinct mutations in the para locus resulted in a decrease in the fraction of cells from which sodium currents could be recorded. This reduction was allele-dependent: macroscopic sodium currents were exhibited in 49% of the neurons in parats1 cultures, 35% in parats2, and only 2% in paraST76. Voltage-clamp experiments demonstrated that the parats2 mutation also affected the gating properties of sodium channels. These results provide convincing evidence that para, a gene recently shown to exhibit sequence similarity to vertebrate sodium channels alpha subunits, encodes functional sodium channels in Drosophila. The finding that one para allele (paraST76) can virtually eliminate the expression of sodium currents strongly argues that the para gene codes for the majority of sodium channels in cultured embryonic neurons.
Authors:
D K O'Dowd; S E Germeraad; R W Aldrich
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuron     Volume:  2     ISSN:  0896-6273     ISO Abbreviation:  Neuron     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1990-04-26     Completed Date:  1990-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8809320     Medline TA:  Neuron     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1301-11     Citation Subset:  IM    
Affiliation:
Department of Neurobiology, Stanford University, California 94305.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Drosophila melanogaster / embryology,  genetics*,  metabolism
Genes
Membrane Potentials
Mutation
Neurons / metabolism
Potassium / metabolism
Sodium / metabolism*
Sodium Channels / metabolism*
Temperature
Grant Support
ID/Acronym/Agency:
NS23294/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Sodium Channels; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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