Document Detail


Alterations in cytoskeletal protein sulfhydryls and cellular glutathione in cultured cells exposed to cadmium and nickel ions.
MedLine Citation:
PMID:  8442019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To understand the mechanisms of Cd2+ and Ni2+ cytotoxicity, we have studied the effects of these two metal ions on the organization of cytoskeletal elements, microtubules (MT) and microfilaments (MF), cytoskeletal protein sulfhydryls and cellular glutathione (GSH) in cultured 3T3 cells. At a metal ion dose that caused 95% inhibition of DNA synthesis, Cd2+ (10 microM, 16 h exposure) induced MT depolymerization whereas Ni2+ (2 mM, 20 h exposure) elicited MT aggregation and bundling. Under these conditions, Cd2+ and Ni2+ also caused MF aggregation and redistribution. Furthermore, exposure of cells to Cd2+ resulted in a dose-dependent increase in cytoskeletal protein sulfhydryls and cellular GSH levels. In contrast, treatment of cells with Ni2+ resulted in a dose-dependent decrease in cytoskeletal protein sulfhydryls as well as cellular GSH content. Time course studies showed that cells exposed to 10 microM Cd2+ exhibited a biphasic response in regulating their cytoskeletal protein sulfhydryls and cellular GSH, e.g. an initial decrease followed by a steady recovery and overshooting upon prolonged incubation. However, restoration of cytoskeletal protein sulfhydryls occurred approximately 2 h after commencement of cellular GSH recovery in Cd(2+)-treated cells. These results suggest that cellular GSH may play an important role in regulating cytoskeletal protein sulfhydryls. On the other hand, decrease of cellular GSH induced by Ni2+ might facilitate oxidation of cytoskeletal protein sulfhydryls and formation of disulfide bonds between individual MT polymers which would favor MT aggregation in Ni(2+)-exposed cells. In addition, we also demonstrated that elevation of cellular GSH in Cd(2+)-treated cells probably resulted from new GSH synthesis.
Authors:
W Li; Y Zhao; I N Chou
Related Documents :
14511129 - Activation of stimulatory heterotrimeric g proteins increases glutathione and protects ...
16278919 - Simultaneous determination of glutathione and reactive oxygen species in individual cel...
17886039 - Coumarin derivatives protection against ros production in cellular models of abeta toxi...
9538239 - Metabolism of 4-hydroxynonenal, a cytotoxic lipid peroxidation product, in thymocytes a...
3378209 - Glutathione contents in human and rodent tumor cells in various phases of the cell cycle.
15832819 - Protection of llc-pk1 cells against hydrogen peroxide-induced cell death by modulation ...
11563879 - The fifth immunoglobulin-like domain of the kit receptor is required for proteolytic cl...
23661569 - Zebularine inhibits the growth of a549 lung cancer cells via cell cycle arrest and apop...
15221529 - Disposal of chloroplasts with abnormal function into the vacuole in arabidopsis thalian...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicology     Volume:  77     ISSN:  0300-483X     ISO Abbreviation:  Toxicology     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-03-30     Completed Date:  1993-03-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  65-79     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Boston University School of Medicine, MA 02118.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Cadmium / toxicity*
DNA / biosynthesis,  drug effects
Dose-Response Relationship, Drug
Glutathione / analysis,  drug effects*
Mice
Microfilaments / drug effects*
Microscopy, Fluorescence
Microtubules / drug effects*
Nickel / toxicity*
Sulfhydryl Compounds / analysis*
Tritium
Grant Support
ID/Acronym/Agency:
R01-ES 03543/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Sulfhydryl Compounds; 10028-17-8/Tritium; 70-18-8/Glutathione; 7440-02-0/Nickel; 7440-43-9/Cadmium; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influence of fatty acid anilides present in toxic oils on the metabolism of exogenous arachidonic ac...
Next Document:  Reduction of liver taurine in rats by beta-alanine treatment increases carbon tetrachloride toxicity...