Document Detail


Alterations in the cell cycle and in the protein level of cyclin D1, p21CIP1, and p16INK4a after exposure to 50 Hz MF in human cells.
MedLine Citation:
PMID:  12201056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of exposure to 50 Hz, 1 mT magnetic fields (MF) on the cell cycle in general, on the DNA synthesis in S-phase, and on the G1-phase regulating proteins Cdk4, cyclin D1, p16INK4a, and p21CIP1 was investigated in human amniotic fluid cells. The BrdU-incorporation assay revealed a significant diminution of S-phase cells in MF-exposed cultures. The protein level of Cdk4 did not change, but MF induced a decreased expression of cyclin D1 after 24 h and 30 h exposures. The level of p16INK4a increased at 1 h and 12 h after exposure, whereas the expression of p21CIP1 was enhanced at 6 h and 12 h after exposure. Reduced levels of both Cdk inhibitors were observed at longer exposure times (24 h, 30 h). Our results suggest an inhibitory effect of MF on the G1-phase induced by altered expression of p16INK4a and p21CIP1.
Authors:
Sandra Lange; Doreen Richard; Torsten Viergutz; Ralf Kriehuber; Dieter G Weiss; Myrtill Simkó
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation and environmental biophysics     Volume:  41     ISSN:  0301-634X     ISO Abbreviation:  Radiat Environ Biophys     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-08-30     Completed Date:  2003-04-02     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  0415677     Medline TA:  Radiat Environ Biophys     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  131-7     Citation Subset:  IM    
Affiliation:
University of Rostock, Institute of Cell Biology and Biosystems Technology, Division of Environmental Physiology, Albert-Einstein-Strasse 3, 18059 Rostock, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amniotic Fluid / cytology,  metabolism,  radiation effects
Cell Cycle / radiation effects
Cell Cycle Proteins / biosynthesis*
Cells, Cultured
Cyclin D1 / biosynthesis
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinases / biosynthesis
Cyclins / biosynthesis
Diploidy
Dose-Response Relationship, Radiation
Electromagnetic Fields*
G1 Phase / radiation effects*
Humans
Proto-Oncogene Proteins*
Reference Values
Reproducibility of Results
S Phase / radiation effects*
Sensitivity and Specificity
Stem Cells / metabolism*,  radiation effects*
Chemical
Reg. No./Substance:
0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Proto-Oncogene Proteins; 136601-57-5/Cyclin D1; EC 2.7.11.22/CDK4 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 4; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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